Evidence map›Paper›PMID 35163006›Full record

ArticleInternational journal of molecular sciences2022

CRISPR Editing Enables Consequential Tag-Activated MicroRNA-Mediated Endogene Deactivation.

Panayiota L Papasavva, Petros Patsali, Constantinos C Loucari, Ryo Kurita, Yukio Nakamura, Marina Kleanthous, Carsten W Lederer

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Panayiota L PapasavvaDepartment of Molecular Genetics Thalassemia, The Cyprus Institute of Neurology and Genetics, Nicosia 2371, Cyprus.ORCID 0000-0003-4410-3119
Petros PatsaliDepartment of Molecular Genetics Thalassemia, The Cyprus Institute of Neurology and Genetics, Nicosia 2371, Cyprus.ORCID 0000-0001-8287-6724
Constantinos C LoucariDepartment of Molecular Genetics Thalassemia, The Cyprus Institute of Neurology and Genetics, Nicosia 2371, Cyprus.
Ryo KuritaResearch and Development Department, Central Blood Institute, Blood Service Headquarters, Japanese Red Cross Society, Koto-ku, Tokyo 135-8521, Japan.
Yukio NakamuraCell Engineering Division, RIKEN BioResource Research Center, Tsukuba 305-0074, Japan.
Marina KleanthousDepartment of Molecular Genetics Thalassemia, The Cyprus Institute of Neurology and Genetics, Nicosia 2371, Cyprus.
Carsten W LedererDepartment of Molecular Genetics Thalassemia, The Cyprus Institute of Neurology and Genetics, Nicosia 2371, Cyprus.ORCID 0000-0003-3920-9584
Cyprus Institute of Neurology and Genetics · CYJapanese Red Cross Society, Japan · JPRIKEN BioResource Research Center · JP

Funding

Eurobank Cyprus CSMM Phd ScholarshipEuropean Commission 306201Norway Grants New infrastructure for diagnosis and treatment of patientsResearch and Innovation Foundation of Cyprus EXCELLENCE/0421/0086Research and Innovation Foundation of Cyprus EXCELLENCE/1216/0092Telethon Cyprus CSMM PhD Scholarship
6 · The paper itself

Abstract

Molecular therapies and functional studies greatly benefit from spatial and temporal precision of genetic intervention. We therefore conceived and explored tag-activated microRNA (miRNA)-mediated endogene deactivation (TAMED) as a research tool and potential lineage-specific therapy. For proof of principle, we aimed to deactivate γ-globin repressor

Indexed as

3' Untranslated RegionsCell LineCRISPR-Cas SystemsDNA End-Joining RepairErythroid CellsGene EditingHEK293 CellsHumansMicroRNAsProof of Concept StudyRepressor Proteins3' Untranslated RegionsBCL11A protein, humanMicroRNAsRepressor ProteinsBCL11ACD34+ cellscell/tissue-type-specific gene therapyerythroid-specificgene tagginghemoglobinopathiesHUDEP-2 cellsγ-globin induction

Identifiers

PMID35163006
PMCPMC8834719
OpenAlexW4206360901

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.