Evidence map›Paper›PMID 35162999›Full record

ArticleInternational journal of molecular sciences2022

Protein Disulfide Isomerase A3 Regulates Influenza Neuraminidase Activity and Influenza Burden in the Lung.

Nicolas Chamberlain, Mona Ruban, Zoe F Mark, Sierra R Bruno, Amit Kumar, Ravishankar Chandrasekaran, Dhemerson Souza De Lima, Danielle Antos, Emily M Nakada, John F Alcorn and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Host-Pathogen Interaction 3.0.International journal of molecular sciences · 2022
    Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Nicolas ChamberlainDepartment of Pathology and Laboratory Medicine, Larner College of Medicine, The University of Vermont, Burlington, VT 05405, USA.
Mona RubanDepartment of Pathology and Laboratory Medicine, Larner College of Medicine, The University of Vermont, Burlington, VT 05405, USA.
Zoe F MarkDepartment of Pathology and Laboratory Medicine, Larner College of Medicine, The University of Vermont, Burlington, VT 05405, USA.
Sierra R BrunoDepartment of Pathology and Laboratory Medicine, Larner College of Medicine, The University of Vermont, Burlington, VT 05405, USA.
Amit KumarDepartment of Pathology and Laboratory Medicine, Larner College of Medicine, The University of Vermont, Burlington, VT 05405, USA.
Ravishankar ChandrasekaranDepartment of Medicine, Larner College of Medicine, The University of Vermont, Burlington, VT 05405, USA.
Dhemerson Souza De LimaDepartment of Pathology and Laboratory Medicine, Larner College of Medicine, The University of Vermont, Burlington, VT 05405, USA.
Danielle AntosDepartment of Pediatrics, Division of Pulmonary Medicine, UPMC Children's Hospital of Pittsburgh, University of Pittsburgh, Pittsburgh, PA 15260, USA.
Emily M NakadaDepartment of Pathology and Laboratory Medicine, Larner College of Medicine, The University of Vermont, Burlington, VT 05405, USA.
John F AlcornDepartment of Pediatrics, Division of Pulmonary Medicine, UPMC Children's Hospital of Pittsburgh, University of Pittsburgh, Pittsburgh, PA 15260, USA.
Vikas AnathyDepartment of Pathology and Laboratory Medicine, Larner College of Medicine, The University of Vermont, Burlington, VT 05405, USA.
University of Vermont · USChildren's Hospital of Pittsburgh · USUniversity of Pittsburgh · US

Funding

Mitochondrial Redox Perturbations in Obese Allergic AsthmaR01HL136917 · NHLBI · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI ANATHY, VIKAS, DIXON, ANNE E · 2018 to 2022
$3.6M
Endoplasmic Reticulum Stress Signaling in Allergen-induced Airway RemodelingR01HL122383 · NHLBI · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI ANATHY, VIKAS · 2015 to 2023
$3.5M
Unfolded protein response in Influenza virus infection and inflammationR01HL141364 · NHLBI · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI ANATHY, VIKAS · 2019 to 2022
$1.6M
NHLBI NIH HHS R01 HL122383NHLBI NIH HHS R01 HL136917NHLBI NIH HHS R01 HL141364
6 · The paper itself

Abstract

Influenza (IAV) neuraminidase (NA) is a glycoprotein required for the viral exit from the cell. NA requires disulfide bonds for proper function. We have recently demonstrated that protein disulfide isomerase (PDI)A3 is required for oxidative folding of IAV hemagglutinin (HA), and viral propagation. However, it not known whether PDIs are required for NA maturation or if these interactions represent a putative target for the treatment of influenza infection. We sought to determine whether PDIA3 is required for disulfide bonds of NA, its activity, and propagation of the virus. Requirement of disulfides for NA oligomerization and activity were determined using biotin switch and redox assays in WT and PDIA3

Indexed as

A549 CellsAnimalsCells, CulturedDisease Models, AnimalDogsEnzyme InhibitorsFemaleHumansInfluenza A Virus, H1N1 SubtypeMadin Darby Canine Kidney CellsMiceNeuraminidaseOrthomyxoviridae InfectionsPrimary Cell CultureProtein Disulfide-IsomerasesProtein FoldingEnzyme InhibitorsNA protein, influenza A virusNeuraminidasePDIA3 protein, humanPdia3 protein, mouseProtein Disulfide-IsomerasesViral ProteinsdisulfidesinfluenzaLOC14NAPDIA3

Identifiers

PMID35162999
PMCPMC8834910
OpenAlexW4205498117

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.