ArticleFoods (Basel, Switzerland)2022
Multiplex PCR Assay for Simultaneous Identification of Five Types of Tuna (
Article in Foods (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Searching for shared epigenetic clocks: evaluating ultra-conserved markers in a de novo genome assembly of the albacore tuna.GeroScience · 2026Article
- Development of a Multiplex PCR Assay for Simultaneous Identification of Six Commercially Important Bivalves.Foods (Basel, Switzerland) · 2025Article
- Fish By-Products Utilization in Food and Health: Extraction Technologies, Bioactive, and Sustainability Challenges.Food science & nutrition · 2025Review
- Comparison of PCR-Based Methods for the Detection of Canned Tuna Species.Journal of food science · 2025Article
- Development of a Rapid and Cost-Effective Multiplex PCR Assay for the Simultaneous Identification of Three Commercially Important Sea Squirt Species (Foods (Basel, Switzerland) · 2025Article
- A universal DNA microarray for rapid fish species authentication.Food chemistry. Molecular sciences · 2025Article
- Development of Single-Nucleotide Polymorphism (SNP)-Based Species-Specific Real-Time PCR Assays for Authenticating Five Highly Priced Tuna.Foods (Basel, Switzerland) · 2024Article
- A Novel Method for Rapid Screening of Salmonidae Ingredients and Accurate Detection of Atlantic Salmon (Molecules (Basel, Switzerland) · 2024Article
- Molecular Rapid Test for Identification of Tuna Species.Biosensors · 2024Article
- Establishment and Application of a Multiplex PCR Assay for Detection of Sclerotium rolfsii, Lasiodiplodia theobromae, and Fusarium oxysporum in Peanut.Molecular biotechnology · 2023Article
- Quality analysis of genomic DNA and authentication of fisheries products based on distinct methods of DNA extraction.PloS one · 2023Article
- Rapid On-Site Identification for Three Arcidae Species (Foods (Basel, Switzerland) · 2022Article
- Development of a multiplex PCR assay for the simultaneous detection of big blue octopus (Food science and biotechnology · 2022Article
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
There is a need to identify the species of similar types of fish, especially those that are commercially sold. Particularly, the price of tuna varies depending on its type, which is difficult to determine as they are sold in cut or processed forms. This study developed a multiplex polymerase chain reaction (PCR) assay to identify the five most common tuna species: bigeye, skipjack, Atlantic bluefin, albacore, and yellowfin tunas. Newly designed species-specific primer sets for these five tuna species were created. Subsequently, the amplicon sizes obtained were 270, 238, 200, 178, and 127 base pairs for bigeye, skipjack, Atlantic bluefin, albacore, and yellowfin tunas, respectively. Each primer's specificity was further tested using 15 other fish species, and no cross-reactivity was observed. To identify multiple targets in a single reaction, multiplex PCR was optimized to increase its resolution and accuracy. The detection levels of the multiplex PCR assay were confirmed to be 1 pg for all the five tunas. Additionally, it was successfully applied to 32 types of commercial tuna products. Therefore, this multiplex PCR assay could be an efficient identification method for various tuna species.
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Registered trials
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