Evidence map›Paper›PMID 35159255›Full record

ArticleCells2022

Acrylonitrile and Pullulan Based Nanofiber Mats as Easily Accessible Scaffolds for 3D Skin Cell Models Containing Primary Cells.

Markus Rimann, Astrid Jüngel, Sara Mousavi, Nicole Moeschlin, Maurizio Calcagni, Karin Wuertz-Kozak, Florian Brunner, Stefan Dudli, Oliver Distler, Christian Adlhart

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 4 countries.

Markus RimannInstitute of Chemistry and Biotechnology (ICBT), ZHAW Zurich University of Applied Sciences, 8820 Wädenswil, Switzerland.ORCID 0000-0002-4605-8190
Astrid JüngelCenter of Experimental Rheumatology, University Hospital Zurich and Balgrist University Hospital,University of Zurich, 8091 Zurich, Switzerland.
Sara MousaviInstitute of Chemistry and Biotechnology (ICBT), ZHAW Zurich University of Applied Sciences, 8820 Wädenswil, Switzerland.ORCID 0000-0002-0011-5305
Nicole MoeschlinInstitute of Chemistry and Biotechnology (ICBT), ZHAW Zurich University of Applied Sciences, 8820 Wädenswil, Switzerland.
Maurizio CalcagniDepartment of Plastic Surgery and Hand Surgery, University Hospital Zurich, University of Zurich, 8091 Zurich, Switzerland.ORCID 0000-0003-4372-0561
Karin Wuertz-KozakDepartment of Biomedical Engineering, Rochester Institute of Technology (RIT), Rochester, NY 14623, USA.ORCID 0000-0002-3281-4629
Florian BrunnerDepartment of Physical Medicine and Rheumatology, Balgrist University Hospital, University of Zurich, 8008 Zurich, Switzerland.ORCID 0000-0002-1420-3824
Stefan DudliCenter of Experimental Rheumatology, University Hospital Zurich and Balgrist University Hospital,University of Zurich, 8091 Zurich, Switzerland.
Oliver DistlerCenter of Experimental Rheumatology, University Hospital Zurich and Balgrist University Hospital,University of Zurich, 8091 Zurich, Switzerland.
Christian AdlhartInstitute of Chemistry and Biotechnology (ICBT), ZHAW Zurich University of Applied Sciences, 8820 Wädenswil, Switzerland.ORCID 0000-0002-4081-221X
University of Zurich · CHZHAW Zurich University of Applied Sciences · CHRochester Institute of Technology · US

Funding

Novartis Foundation for medical-biological research 18B082University Medicine Zurich / Hochschulmedizin Zurich, SKINTEGRITYZHAW Dept. N grant, Biomat@N
6 · The paper itself

Abstract

(1) Background: Three-dimensional (3D) collagen I-based skin models are commonly used in drug development and substance testing but have major drawbacks such as batch-to-batch variations and ethical concerns. Recently, synthetic nanofibrous scaffolds created by electrospinning have received increasing interest as potential alternatives due to their morphological similarities to native collagen fibrils in size and orientation. The overall objective of this proof-of-concept study was to demonstrate the suitability of two synthetic polymers in creating electrospun scaffolds for 3D skin cell models. (2) Methods: Electrospun nanofiber mats were produced with (i) poly(acrylonitrile-co-methyl acrylate) (P(AN-MA)) and (ii) a blend of pullulan (Pul), poly(vinyl alcohol) (PVA) and poly(acrylic acid) (PAA) (Pul/PVA/PAA) and characterized by scanning electron microscopy (SEM) and diffuse reflectance infrared Fourier transform (DRIFT) spectra. Primary skin fibroblasts and keratinocytes were seeded onto the nanofiber mats and analyzed for phenotypic characteristics (phalloidin staining), viability (Presto Blue HS assay), proliferation (Ki-67 staining), distribution (H/E staining), responsiveness to biological stimuli (qPCR), and formation of skin-like structures (H/E staining). (3) Results: P(AN-MA) mats were more loosely packed than the Pul/PVA/PAA mats, concomitant with larger fiber diameter (340 nm ± 120 nm vs. 250 nm ± 120 nm,

Indexed as

AcrylonitrileNanofibersCollagenGlucansTissue EngineeringTissue ScaffoldsAcrylonitrileCollagenGlucanspullulan3D cell culturealternative methodsbiomaterialmicroenvironmenttissue engineering

Identifiers

PMID35159255
PMCPMC8834075
OpenAlexW4210256944

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.