Evidence map›Paper›PMID 35159253›Full record

ArticleCells2022

Cryptococcal Protease(s) and the Activation of SARS-CoV-2 Spike (S) Protein.

Nozethu Mjokane, Maphori Maliehe, Olufemi S Folorunso, Adepemi O Ogundeji, Onele M N Gcilitshana, Jacobus Albertyn, Carolina H Pohl, Olihile M Sebolai

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Nozethu MjokaneDepartment of Microbiology and Biochemistry, University of the Free State, 205 Nelson Mandela Drive, Park West, Bloemfontein 9301, South Africa.ORCID 0000-0002-2915-4543
Maphori MalieheDepartment of Microbiology and Biochemistry, University of the Free State, 205 Nelson Mandela Drive, Park West, Bloemfontein 9301, South Africa.
Olufemi S FolorunsoDepartment of Microbiology and Biochemistry, University of the Free State, 205 Nelson Mandela Drive, Park West, Bloemfontein 9301, South Africa.ORCID 0000-0002-0485-2273
Adepemi O OgundejiDepartment of Microbiology and Biochemistry, University of the Free State, 205 Nelson Mandela Drive, Park West, Bloemfontein 9301, South Africa.ORCID 0000-0002-7886-0524
Onele M N GcilitshanaDepartment of Microbiology and Biochemistry, University of the Free State, 205 Nelson Mandela Drive, Park West, Bloemfontein 9301, South Africa.ORCID 0000-0003-0286-8578
Jacobus AlbertynDepartment of Microbiology and Biochemistry, University of the Free State, 205 Nelson Mandela Drive, Park West, Bloemfontein 9301, South Africa.
Carolina H PohlDepartment of Microbiology and Biochemistry, University of the Free State, 205 Nelson Mandela Drive, Park West, Bloemfontein 9301, South Africa.ORCID 0000-0001-6928-5663
Olihile M SebolaiDepartment of Microbiology and Biochemistry, University of the Free State, 205 Nelson Mandela Drive, Park West, Bloemfontein 9301, South Africa.ORCID 0000-0002-0182-1084

Funding

National Research Foundation of South Africa grant no. 137965
6 · The paper itself

Abstract

In this contribution, we report on the possibility that cryptococcal protease(s) could activate the SARS-CoV-2 spike (S) protein. The S protein is documented to have a unique four-amino-acid sequence (underlined, SPRRAR↓S) at the interface between the S1 and S2 sites, that serves as a cleavage site for the human protease, furin. We compared the biochemical efficiency of cryptococcal protease(s) and furin to mediate the proteolytic cleavage of the S1/S2 site in a fluorogenic peptide. We show that cryptococcal protease(s) processes this site in a manner comparable to the efficiency of furin (

Indexed as

Amino Acid SequenceAspartic Acid ProteasesBacterial ProteinsBinding SitesCOVID-19Cryptococcus neoformansFluorescent DyesFurinHumansPandemicsPeptidesProteolysisSARS-CoV-2Spike Glycoprotein, CoronavirusAspartic Acid ProteasesBacterial ProteinsFluorescent DyesFurinPeptidesSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2cryptococcal infectionCryptococcusCryptococcus neoformansfluorogenic peptidefurinproteaseproteolytic cleavageS1/S2 cleavage siteSARS-CoV-2spike (S) protein

Identifiers

PMID35159253
PMCPMC8834071

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.