ArticleFrontiers in veterinary science2022
Development of a One-Step Multiplex Real-Time PCR Assay for the Detection of Viral Pathogens Associated With the Bovine Respiratory Disease Complex.
Article in Frontiers in veterinary science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 26 citations in OpenAlex.
- Development of an RT-RAA-CRISPR/Cas12a fluorescent detection method for the detection of bovine viral diarrhea virus 1.BMC veterinary research · 2026Article
- Development and epidemiological investigation of a TaqMan-based multiplex real-time quantitative PCR assay for simultaneous detection of five bovine viruses (BVDV, AKAV, BNoV, BEV, and BCoV).Frontiers in veterinary science · 2026Article
- Quality control strategy for differentiating induced pluripotent stem cells into pancreatic β-cells before transplantation.Regenerative medicine · 2026Review
- Molecular investigation and viral load analysis of bovine respiratory syncytial virus in cattle with bovine respiratory disease complex in Inner Mongolia, China.Frontiers in cellular and infection microbiology · 2026Article
- Development and validation of a multiplex TaqMan real-time quantitative PCR assay for the simultaneous detection of six major bacterial respiratory pathogens in goats.Frontiers in veterinary science · 2026Article
- Next-generation detection in bovine respiratory and enteric diseases: metagenomic and amplicon sequencing insights into microbial diversity.Frontiers in veterinary science · 2026Review
- A Comprehensive Review: Molecular Diagnostics and Multi-Omics Approaches to Understanding Bovine Respiratory Disease.Veterinary sciences · 2025Review
- Application of Bovine Nasal Epithelial Cells as an In Vitro Model for Studying Viral Infection in the Upper Respiratory Tract.Viruses · 2025Article
- Development of a Novel, Non-Invasive Saliva Sampling Method for the Detection of Bovine Respiratory Viruses.Veterinary sciences · 2025Article
- Field-validated multiplex RT-qPCR for simultaneous detection of bovine respiratory syncytial virus and bovine parainfluenza virus-3 in bovine respiratory samples.Frontiers in veterinary science · 2025Article
- Establishment of a multiplex qPCR assay for the detection of pathogens associated with bovine respiratory disease complex.Frontiers in veterinary science · 2025Article
- Establishment of a TaqMan qPCR method with MGB probe for the specific detection of BVDV field strains circulating in China.Frontiers in veterinary science · 2025Article
- Research progress on the diagnostic techniques and vaccine development of bovine herpesvirus types 1.Frontiers in veterinary science · 2025Review
- Direct TaqMan assay for the detection and genotyping of bovine viral diarrhea virus types 1 and 2.Archives of virology · 2024Article
- Rapid detection of causative bacteria including multiple infections of bovine respiratory disease using 16S rRNA amplicon-based nanopore sequencing.Veterinary research communications · 2024Article
- Early detection of bovine respiratory disease in pre-weaned dairy calves using sensor based feeding, movement, and social behavioural data.Scientific reports · 2024Article
- Bovine Parainfluenza-3 Virus Detection Methods and Prevalence in Cattle: A Systematic Review and Meta-Analysis.Animals : an open access journal from MDPI · 2024Review
- Diagnosis of bovine viral diarrhea virus: an overview of currently available methods.Frontiers in microbiology · 2024Review
- Development of a pan-genotypic monoclonal antibody-based competitive ELISA for the detection of antibodies against Bovine viral diarrhea virus.Frontiers in immunology · 2024Article
- A Rapid and Novel Multiplex PCR Assay for Simultaneous Detection of Multiple Viruses Associated with Bovine Gastroenteritis.Indian journal of microbiology · 2023Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bovine respiratory disease complex (BRDC) occurs widely in cattle farms. The main viral pathogens include bovine viral diarrhea virus (BVDV), Bovine herpesvirus 1 (BoHV-1), bovine parainfluenza virus type 3 (BPIV3), and bovine respiratory syncytial virus (BRSV), and the newly emerged influenza D virus (IDV). In this study, we have developed a one-step multiplex real-time Polymerase Chain Reaction (PCR) capable of simultaneously detecting these five viral pathogens causing BRDC. The established assay could specifically detect targeted viruses without cross-reaction with others. The detection limit was ~10 copies/reaction for single real-time PCR and 100 copies/ reaction for multiplex real-time PCR assay. A total of 213 nasal samples from cattle with signs of respiratory tract disease were then collected for performance evaluation of the established platform, proving that the method has good specificity and sensitivity. The surveillance data suggested that BVDV and BoHV-1 infections are the dominant cause of BRDC in the herd, whereas the detection rate of IDV, BIPV3, and BRSV is relatively lower. In summary, the established assay provides technical support for rapid clinical detection of BRDC associated viral pathogens to guide the formulation of BRDC prevention and control measures.
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