ArticleFrontiers in bioengineering and biotechnology2021
CRISPR Interference Modules as Low-Burden Logic Inverters in Synthetic Circuits.
Article in Frontiers in bioengineering and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed.
- Harnessing CRISPR interference to resensitize laboratory strains and clinical isolates to last resort antibiotics.Scientific reports · 2025Article
- A synthetic biology toolkit for rationally designing genetic circuits inFrontiers in systems biology · 2025Article
- Construction of multilayered gene circuits using de-novo-designed synthetic transcriptional regulators in cell-free systems.Journal of biological engineering · 2024Article
- Synthetic genetic oscillators demonstrate the functional importance of phenotypic variation in pneumococcal-host interactions.Nature communications · 2023Article
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9 authors.
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Abstract
CRISPR and CRISPRi systems have revolutionized our biological engineering capabilities by enabling the editing and regulation of virtually any gene, via customization of single guide RNA (sgRNA) sequences. CRISPRi modules can work as programmable logic inverters, in which the dCas9-sgRNA complex represses a target transcriptional unit. They have been successfully used in bacterial synthetic biology to engineer information processing tasks, as an alternative to the traditionally adopted transcriptional regulators. In this work, we investigated and modulated the transfer function of several model systems with specific focus on the cell load caused by the CRISPRi logic inverters. First, an optimal expression cassette for dCas9 was rationally designed to meet the low-burden high-repression trade-off. Then, a circuit collection was studied at varying levels of dCas9 and sgRNAs targeting three different promoters from the popular
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