ArticleBiomedical optics express2022
Aberration measurement and correction on a large field of view in fluorescence microscopy.
Article in Biomedical optics express, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 24 citations in OpenAlex.
- Hadamard based single-pixel microscopy using sensor-less adaptive optics supported by multi-actuator adaptive lens.Nature communications · 2025Article
- Recent Progress in Liquid Microlenses and Their Arrays for Adaptive and Applied Optical Systems.Micromachines · 2025Review
- Wavefront estimation through structured detection in laser scanning microscopy.Biomedical optics express · 2025Article
- Practical guidelines for cell segmentation models under optical aberrations in microscopy.Computational and structural biotechnology journal · 2024Article
- Distortion Correction and Denoising of Light Sheet Fluorescence Images.Sensors (Basel, Switzerland) · 2024Article
- Tandem aberration correction optics (TACO) in wide-field structured illumination microscopy.Biomedical optics express · 2023Article
- Multiplex imaging in immuno-oncology.Journal for immunotherapy of cancer · 2023Review
- Blind deconvolution in autocorrelation inversion for multiview light-sheet microscopy.Microscopy research and technique · 2022Article
- Image Reconstruction in Light-Sheet Microscopy: Spatially Varying Deconvolution and Mixed Noise.Journal of mathematical imaging and vision · 2022Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aberrations induced by the sample and/or by the sample holder limit the resolution of optical microscopes. Wavefront correction can be achieved using a deformable mirror with wavefront sensorless optimization algorithms but, despite the complexity of these systems, the level of correction is often limited to a small area in the field of view of the microscope. In this work, we present a plug and play module for aberration measurement and correction. The wavefront correction is performed through direct wavefront reconstruction using the spinning-pupil aberration measurement and controlling a deformable lens in closed loop. The lens corrects the aberrations in the center of the field of view, leaving residual aberrations at the margins, that are removed by anisoplanatic deconvolution. We present experimental results obtained in fluorescence microscopy, with a wide field and a light sheet fluorescence microscope. These results indicate that detection and correction over the full field of view can be achieved with a compact transmissive module placed in the detection path of the fluorescence microscope.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.