ArticleMycoKeys2022
A long-read amplicon approach to scaling up the metabarcoding of lichen herbarium specimens.
Article in MycoKeys, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Whole genome sequencing of historical specimens from the world's largest fungal collection yields high-quality assemblies.The New phytologist · 2026Article
- The First Whole Genome Sequencing of Historical Lichen Specimens Enables Genome-Wide Analysis of Fungal and Algal Symbionts.Ecology and evolution · 2025Article
- Surveying lichen diversity in forests: A comparison of expert mapping and eDNA metabarcoding of bark surfaces.MycoKeys · 2024Article
- Does One Size Fit All? Variations in the DNA Barcode Gaps of Macrofungal Genera.Journal of fungi (Basel, Switzerland) · 2023Article
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Authors and funding
2 authors.
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Abstract
Reference sequence databases are critical to the accurate detection and identification of fungi in the environment. As repositories of large numbers of well-curated specimens, herbaria and fungal culture collections have the material resources to generate sequence data for large number of taxa, and could therefore allow filling taxonomic gaps often present in reference sequence databases. Financial resources to do that are however often lacking, so that recent efforts have focused on decreasing sequencing cost by increasing the number of multiplexed samples per sequencing run while maintaining high sequence quality. Following a previous study that aimed at decreasing sequencing cost for lichen specimens by generating fungal ITS barcodes for 96 specimens using PacBio amplicon sequencing, we present a method that further decreases lichen specimen metabarcoding costs. A total of 384 mixed DNA extracts obtained from lichen herbarium specimens, mostly from the four genera
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