ArticleProceedings of the National Academy of Sciences of the United States of America1986
Biogenesis of the nuclear lamina: in vivo synthesis and processing of nuclear protein precursors.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
- Farnesylated prelamin A induces fibroblast polarity defects in premature aging disorders by inhibiting nesprin-2-SUN2 LINC complex function.Journal of cell science · 2026Article
- Deleterious assembly of the lamin A/C mutant p.S143P causes ER stress in familial dilated cardiomyopathy.Journal of cell science · 2016Article
- Nucleoplasmic Lamin A/C and Polycomb group of proteins: An evolutionarily conserved interplay.Nucleus (Austin, Tex.) · 2016Article
- AKT1-mediated Lamin A/C degradation is required for nuclear degradation and normal epidermal terminal differentiation.Cell death and differentiation · 2015Article
- Nuclear lamins.Cold Spring Harbor perspectives in biology · 2010Review
- Analysis of prelamin A biogenesis reveals the nucleus to be a CaaX processing compartment.Molecular biology of the cell · 2008Article
- Type B lamins remain associated with the integral nuclear envelope protein p58 during mitosis: implications for nuclear reassembly.The EMBO journal · 1994Article
- The role of CaaX-dependent modifications in membrane association of Xenopus nuclear lamin B3 during meiosis and the fate of B3 in transfected mitotic cells.The Journal of cell biology · 1993Article
- Phosphorylation on protein kinase C sites inhibits nuclear import of lamin B2.The Journal of cell biology · 1993Article
- Nucleoplasmic localization of prelamin A: implications for prenylation-dependent lamin A assembly into the nuclear lamina.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- The CaaX motif is required for isoprenylation, carboxyl methylation, and nuclear membrane association of lamin B2.The Journal of cell biology · 1991Article
- Isoprenylation is required for the processing of the lamin A precursor.The Journal of cell biology · 1990Article
- Modification of nuclear lamin proteins by a mevalonic acid derivative occurs in reticulocyte lysates and requires the cysteine residue of the C-terminal CXXM motif.The EMBO journal · 1989Article
- The conserved carboxy-terminal cysteine of nuclear lamins is essential for lamin association with the nuclear envelope.The Journal of cell biology · 1989Article
- Interconversion of Drosophila nuclear lamin isoforms during oogenesis, early embryogenesis, and upon entry of cultured cells into mitosis.The Journal of cell biology · 1989Article
- Incorporation of a product of mevalonic acid metabolism into proteins of Chinese hamster ovary cell nuclei.The Journal of cell biology · 1988Article
- A major 62-kD intranuclear matrix polypeptide is a component of metaphase chromosomes.The Journal of cell biology · 1988Article
- Drosophila nuclear lamin precursor Dm0 is translated from either of two developmentally regulated mRNA species apparently encoded by a single gene.The Journal of cell biology · 1988Article
- Nuclear lamin LI of Xenopus laevis: cDNA cloning, amino acid sequence and binding specificity of a member of the lamin B subfamily.The EMBO journal · 1987Article
- Biosynthesis and interconversion of Drosophila nuclear lamin isoforms during normal growth and in response to heat shock.The Journal of cell biology · 1987Article
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Utilizing antibodies against lamins A, B1, and B2, we have studied the biogenesis of the nuclear lamina in chicken embryo fibroblasts. (Lamins B1 and B2 have been identified recently as structurally distinct "lamin B" proteins.) We demonstrate that, unique among the nuclear proteins studied to date, lamin A is synthesized as a higher molecular mass precursor. A short-lived higher molecular mass variant (t 1/2 approximately equal to 3 min) accompanying the mature-size protein was also detected in the case of lamin B2 biosynthesis, but no precursor was found for lamin B1. By combining pulse-chase experiments with subcellular fractionation, we provide evidence that synthesis of lamin proteins occurs on free polysomes; subsequently, the newly synthesized proteins become rapidly associated with a crude nuclear fraction. The lamin A precursor is processed within the nucleus with a half-time of about 30 min. Concomitantly, lamin proteins acquire a characteristic resistance to detergent extraction, suggesting their insertion into a submembraneous protein network. The described biogenetic pathway involving precursor synthesis and processing is very unusual for nuclear proteins; it may have interesting implications for the mechanisms of transport and assembly of poorly soluble nuclear proteins.
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