Evidence map›Paper›PMID 35152764›Full record

ArticlePhilosophical transactions. Series A, Mathematical, physical, and engineering sciences2022

Super-resolution microscopy: a brief history and new avenues.

Kirti Prakash, Benedict Diederich, Rainer Heintzmann, Lothar Schermelleh

Abstract read
In one paragraph

Article in Philosophical transactions. Series A, Mathematical, physical, and engineering sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed.

  1. Article
  2. Article
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  4. Absolute quantification of fluorescent protein fusions by mass spectrometry.Protein science : a publication of the Protein Society · 2026
    Article
  5. Article
  6. Article
  7. Review
  8. Review
  9. Review
  10. Perspective: An outlook on fluorescence tracking.The Journal of chemical physics · 2025
    Article
  11. Article
  12. Review
  13. Review
  14. Fluid Flow Measurements in Nanoslits Using Holographic Microscopy.Langmuir : the ACS journal of surfaces and colloids · 2025
    Article
  15. Article
  16. Article
  17. Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Kirti PrakashIntegrated Pathology Unit, Centre for Molecular Pathology, The Royal Marsden Trust and Institute of Cancer Research, Sutton SM2 5NG, UK.ORCID 0000-0002-0325-9988
Benedict DiederichLeibniz Institute for Photonic Technology, Albert-Einstein-Strasse 9, 07745 Jena, Germany.ORCID 0000-0003-0453-6286
Rainer HeintzmannLeibniz Institute for Photonic Technology, Albert-Einstein-Strasse 9, 07745 Jena, Germany.ORCID 0000-0002-4950-1936
Lothar SchermellehDepartment of Biochemistry, University of Oxford, Oxford OX1 3QU, UK.

Funding

Wellcome Trust
6 · The paper itself

Abstract

Super-resolution microscopy (SRM) is a fast-developing field that encompasses fluorescence imaging techniques with the capability to resolve objects below the classical diffraction limit of optical resolution. Acknowledged with the Nobel prize in 2014, numerous SRM methods have meanwhile evolved and are being widely applied in biomedical research, all with specific strengths and shortcomings. While some techniques are capable of nanometre-scale molecular resolution, others are geared towards volumetric three-dimensional multi-colour or fast live-cell imaging. In this editorial review, we pick on the latest trends in the field. We start with a brief historical overview of both conceptual and commercial developments. Next, we highlight important parameters for imaging successfully with a particular super-resolution modality. Finally, we discuss the importance of reproducibility and quality control and the significance of open-source tools in microscopy. This article is part of the Theo Murphy meeting issue 'Super-resolution structured illumination microscopy (part 2)'.

Indexed as

Image Processing, Computer-AssistedOptical ImagingMicroscopy, FluorescenceReproducibility of Resultscomputational imagingfrugal microscopyimage processingspatial resolutionstructured illumination microscopysuper-resolution microscopy

Identifiers

PMID35152764
PMCPMC8841785

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.