ArticleThe New phytologist2022
Dimerization of PtrMYB074 and PtrWRKY19 mediates transcriptional activation of PtrbHLH186 for secondary xylem development in Populus trichocarpa.
Article in The New phytologist, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 66 citations in OpenAlex.
- A MYB-Related Transcriptional Module Cooperatively Represses Xylem Development in Poplar.Plant biotechnology journal · 2026Article
- A MYB-WRKY feedback module activates MdAAT2‑like to regulate aromatic ester biosynthesis during apple ripening.Molecular horticulture · 2026Article
- Genome-Wide Identification and Characterization of bHLH Family inLife (Basel, Switzerland) · 2026Article
- Establishment of a protoplast isolation and transient transformation system for tung tree (Vernicia fordii).Plant methods · 2026Article
- TaWRKY115 enhances cold tolerance by weakening the expression of TaMYB4 on CBFs in common wheat.National science review · 2026Article
- WRKY27-RAP2.7 Regulatory Module Promotes Cold Tolerance via Modulation of Lignin Biosynthesis and Redox Homeostasis by Regulating Cinnamyl Alcohol Dehydrogenase 7 and Glutathione S-Transferase F6.Plant biotechnology journal · 2026Article
- Whole genome identification and operational analysis of theFrontiers in plant science · 2026Article
- Article
- Overexpression of a Xylem-Dominant Expressing BTB Gene,Plants (Basel, Switzerland) · 2025Article
- Populus PtrbHLH011 Is a Transcriptional Co-Regulator Involved in the Activation of Cell Wall Biosynthesis by Iron Deprivation.Plant biotechnology journal · 2025Article
- PagMIR166c targets PagECH2 to regulate cambial differentiation into secondary xylem, cell expansion, and lignin deposition via different auxin signaling pathways in poplar stems.The New phytologist · 2025Article
- Natural variation in PtobZIP18 confers the trade-off between stem growth and drought tolerance in Populus.Plant biotechnology journal · 2025Article
- The role of PagC3H3 in lignin biosynthesis and insect resistance in Populus alba × Populus glandulosa.Plant cell reports · 2025Article
- Bioengineered poplar fibres via PagGLR2.8 editing: A synergistic design for high-performance biocomposites.Plant biotechnology journal · 2025Article
- PtrVINV2 is dispensable for cellulose synthesis but essential for salt tolerance in Populus trichocarpa Torr. and Gray.Plant biotechnology journal · 2025Article
- Genome-wide identification of five fern bHLH families and functional analysis of bHLHs in lignin biosynthesis in Alsophila spinulosa.BMC genomics · 2025Article
- Epigenetic regulation of lignin biosynthesis in wood formation.The New phytologist · 2025Article
- From chromatin to crop: epigenetic innovations in bioenergy systems.Frontiers in plant science · 2025Review
- Identification of key genes involved in lignin and flavonoid accumulation during Tilia tuan seed maturation.Plant cell reports · 2024Article
- ELONGATED HYPOCOTYL 5a modulates FLOWERING LOCUS T2 and gibberellin levels to control dormancy and bud break in poplar.The Plant cell · 2024Article
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wood formation is controlled by transcriptional regulatory networks (TRNs) involving regulatory homeostasis determined by combinations of transcription factor (TF)-DNA and TF-TF interactions. Functions of TF-TF interactions in wood formation are still in the early stages of identification. PtrMYB074 is a woody dicot-specific TF in a TRN for wood formation in Populus trichocarpa. Here, using yeast two-hybrid and bimolecular fluorescence complementation, we conducted a genome-wide screening for PtrMYB074 interactors and identified 54 PtrMYB074-TF pairs. Of these pairs, 53 are novel. We focused on the PtrMYB074-PtrWRKY19 pair, the most highly expressed and xylem-specific interactor, and its direct transregulatory target, PtrbHLH186, the xylem-specific one of the pair's only two direct TF target genes. Using transient and CRISPR-mediated transgenesis in P. trichocarpa coupled with chromatin immunoprecipitation and electrophoretic mobility shift assays, we demonstrated that PtrMYB074 is recruited by PtrWRKY19 and that the PtrMYB074-PtrWRKY19 dimers are required to transactive PtrbHLH186. Overexpressing PtrbHLH186 in P. trichocarpa resulted in retarded plant growth, increased guaiacyl lignin, a higher proportion of smaller stem vessels and strong drought-tolerant phenotypes. Knowledge of the PtrMYB074-PtrWRKY19-PtrbHLH186 regulation may help design genetic controls of optimal growth and wood formation to maximize beneficial wood properties while minimizing negative effects on growth.
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