Evidence map›Paper›PMID 35152387›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

In Situ Observation of Abscisic Acid Distribution in Major Crop Species by Immunofluorescence Labeling.

Mo-Xian Chen, Chong-Chong Lu, Jianhua Zhang, Ying-Gao Liu

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.5field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 2 countries.

Mo-Xian ChenCo-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Jiangsu Province, China.
Chong-Chong LuState Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University, Taian, Shandong, China.
Jianhua ZhangDepartment of Biology, Hong Kong Baptist University, and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
Ying-Gao LiuState Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University, Taian, Shandong, China. liuyg@sdau.edu.cn.
Shandong Agricultural University · CNChinese Academy of Sciences · CNChinese University of Hong Kong · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Immunofluorescent approach uses fluorophore-conjugated antibodies to target molecules of interest for the observation of their distribution and microenvironment at cellular or tissue level. In connection with modern fluorescence microscopy, immunofluorescence has high sensitivity and specificity, resulting in a wide range of applications in animal studies. However, the protocols of immunofluorescence are seldom reported. To this end, we describe an optimized protocol for the detection of plant hormone abscisic acid (ABA) in monocot crop species, including maize and rice. By using immunofluorescence technique provided from this protocol, investigation of ABA distribution will continue to deeper our insights in crop developments and stress responses.

Indexed as

Abscisic AcidOryzaFluorescent Antibody TechniqueGene Expression Regulation, PlantPlant ProteinsZea maysAbscisic AcidPlant ProteinsAbscisic acidFluorescence microscopyImmunofluorescenceOryza sativaTissue imagingZea mays

Identifiers

PMID35152387
OpenAlexW4213105890

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.