ArticleMethods in molecular biology (Clifton, N.J.)2022
Screening of ABA Transporters by a Yeast Two-Hybrid System-Based Screening Using the Receptor Complex as a Sensor.
Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed, 4 citations in OpenAlex.
- The Arabidopsis NPF7.2 mediates coumarin uptake for root iron acquisition.The New phytologist · 2026Article
- Molecular profiling ofFrontiers in plant science · 2025Article
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Authors and funding
3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Abscisic acid (ABA) is a plant hormone that is involved in many physiological events and is present in most plant tissues. How ABA moves within plants has not been well understood. To characterize the physiological consequences as well as the underlying molecular events responsible for ABA movement, it is crucial to identify the transporter proteins. Several approaches have been successful in identifying ABA transporters. In this chapter, we outline a methodology to directly identify proteins capable of transporting ABA in a heterologous yeast system. In our assay, the ABA receptor [PYRABACTIN RESISTANCE1 (PYR1) and PYR1-LIKE (PYL), also known as REGULATORY COMPONENTS OF ABA RECEPTOR (RCAR)] and the protein phosphatases of type 2C (PP2C) coreceptor interact in an ABA-dependent manner. A yeast two-hybrid (Y2H) system is used to monitor interactions between the receptor and PP2C and, hence, the ABA concentration in the yeast cells. Screening cDNAs that induce or inhibit the receptor-PP2C interaction in the presence of ABA allows us to identify candidate transporters. ABA transport activities of the putative transporter proteins can be validated by quantifying hormone levels in the yeast cells using liquid chromatography tandem-mass spectrometry (LC-MS/MS).
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