Evidence map›Paper›PMID 35152376›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Inhibition of Type 2C Protein Phosphatases by ABA Receptors in Abscisic Acid-Mediated Plant Stress Responses.

Yeongmok Lee, Suhyeon Heo, Sangho Lee

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.1field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Yeongmok LeeDepartment of Biological Sciences, Sungkyunkwan University, Suwon, South Korea.
Suhyeon HeoDepartment of Biological Sciences, Sungkyunkwan University, Suwon, South Korea.
Sangho LeeDepartment of Biological Sciences, Sungkyunkwan University, Suwon, South Korea. sangholee@skku.edu.
Sungkyunkwan University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Abscisic acid (ABA) signaling pathway constitutes a key stress response mechanism in plants. Alternate interaction of a type 2C protein phosphatase (PP2C) with either an ABA receptor or a SNF1-related protein kinase 2 (SnRK2) is at the core of regulation of the ABA signaling pathway. Binding of an ABA receptor to a PP2C inhibits the enzymatic activity of the PP2C. Presence of multiple paralogs of ABA receptors and PP2Cs necessitates development of an inhibition assay. Here, we describe how to prepare the recombinant proteins of ABA receptors and PP2Cs for the inhibition assay and how to perform a colorimetry-based inhibition assay.

Indexed as

ArabidopsisArabidopsis ProteinsAbscisic AcidGene Expression Regulation, PlantPhosphoprotein PhosphatasesProtein Phosphatase 2CAbscisic AcidArabidopsis ProteinsPhosphoprotein PhosphatasesProtein Phosphatase 2CABA-dependent inhibitionABA receptorABA signalingAbscisic acidPhosphatase inhibition assaypNPPPP2C

Identifiers

PMID35152376
OpenAlexW4213331048

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.