Evidence map›Paper›PMID 35149935›Full record

ArticleMolecular biology reports2022

Molecular cloning, characterization and expression analysis of two 12-oxophytodienoate reductases (NtOPR1 and NtOPR2) from Nicotiana tabacum.

Feiyan Huang, Farhat Abbas, Dylan O'Neill Rothenberg, Muhammad Imran, Sajid Fiaz, Naveed Ur Rehman, Sikandar Amanullah, Afifa Younas, Yan Ding, Xianjie Cai and 6 more

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Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed, 2 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

16 authors at 6 institutions in 2 countries.

Feiyan HuangCollege of Agriculture and Life Sciences, Yunnan Urban Agricultural Engineering & Technological Research Center, Kunming University, Kunming, China.
Farhat AbbasThe Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China.ORCID http://orcid.org/0000-0003-3704-5213
Dylan O'Neill RothenbergCollege of Horticulture Science, South China Agricultural University, Guangzhou, 510642, China.
Muhammad ImranDepartment of Crop Science and Technology, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
Sajid FiazDepartment of Plant Breeding and Genetics, University of Haripur, Khyber Pakhtunkhwa, Pakistan.
Naveed Ur RehmanState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China.
Sikandar AmanullahCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China.
Afifa YounasDepartment of Botany, Lahore College for Women University, Lahore, Pakistan.
Yan DingMaterial Procurement Center, Shanghai Tobacco Group Co., Ltd, Shanghai, 200082, Yunnan, China.
Xianjie CaiMaterial Procurement Center, Shanghai Tobacco Group Co., Ltd, Shanghai, 200082, Yunnan, China. caixj@sh.tobacco.com.cn.
Xiaolong ChenTobacco Leaf Purchase Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450000, China.
Lei YuCollege of Agriculture and Life Sciences, Yunnan Urban Agricultural Engineering & Technological Research Center, Kunming University, Kunming, China.
Xianwen YeKunming Tobacco Corporation of Yunnan Province, Kunming, 650021, China.
Lin JiangHonghe Tobacco Corporation of Yunnan Province, Honghe, 661400, China.
Yanguo KeCollege of Agriculture and Life Sciences, Yunnan Urban Agricultural Engineering & Technological Research Center, Kunming University, Kunming, China. keyanguo@126.com.
Yuansheng HeLincang Company of Yunnan Tobacco Company, Lincang, 677000, China.
China Tobacco · CNSouth China Agricultural University · CNKunming University · CNLahore College for Women University · PKNortheast Agricultural University · CNUniversity of Haripur · PK

Funding

Yunnan Provincial Science and Technology Department 2018530000241016Yunnan Provincial Science and Technology Department 2018530000241020Yunnan Provincial Science and Technology Department 2019530000241011Yunnan Provincial Science and Technology Department 2019530000241028Yunnan Provincial Science and Technology Department 2021J0716
6 · The paper itself

Abstract

background12-oxophytodienoic acid (OPDA) is a signaling molecule involved in defense and stress responses in plants. 12-oxophytodienoate reductase (OPR) is involved in the biosynthesis of jasmonic acid and trigger the conversion of OPDA into 3-oxo-2(2'[Z]-pentenyl)-cyclopentane-1-octanoic acid (OPC-8:0). METHODS AND

resultsSequence analysis revealed that Nicotiana tabacum 12-oxophytodienoate reductase 1 (OPR1) and OPR2 encoded polypeptides of 375 and 349 amino acids with molecular masses of 41.67 and 39.04 kilodaltons (kDa), respectively, while the deduced protein sequences of NtOPR1 and NtOPR2 showed high homology with other 12-oxophytodienoate reductases. BLAST (Basic local alignment search tool) analysis revealed that both NtOPRs belong to the family of Old Yellow Enzymes (OYE), and analysis of genomic DNA structure indicated that both genes include 5 exons and 4 introns. Phylogenetic analysis using MEGA X showed that NtOPR1 and NtOPR2 shared a close evolutionary relationship with Nicotiana attenuata 12-oxophytodienoate reductases. In silico analysis of subcellular localization indicated the probable locations of NtOPR1 and NtOPR2 to be the cytoplasm and the peroxisome, respectively. Tissue-specific expression assays via qRT-PCR revealed that NtOPR1 and NtOPR2 genes were highly expressed in Nicotiana tabacum roots, temperately expressed in leaves and flowers, while low expression was observed in stem tissue.

conclusionsPresently, two 12-oxophytodienoate reductase genes (NtOPR1 and NtOPR2) were cloned and comprehensively characterized. Our findings provide comprehensive analyses that may guide future deep molecular studies of 12-oxophytodienoate reductases in Nicotiana tabacum.

Indexed as

NicotianaOxidoreductases Acting on CH-CH Group DonorsCloning, MolecularFatty Acids, UnsaturatedPhylogeny12-oxophytodienoate reductase12-oxophytodienoic acidFatty Acids, UnsaturatedOxidoreductases Acting on CH-CH Group Donors12-oxophytodienoate reductaseCharacterizationExpression profilePhylogenyTobacco

Identifiers

PMID35149935
OpenAlexW4211167458

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.