ArticleCommunications biology2022
Adaptive laboratory evolution in S. cerevisiae highlights role of transcription factors in fungal xenobiotic resistance.
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Article in Communications biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15citing papers in PubMed
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15 citing papers in PubMed, 21 citations in OpenAlex.
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Authors and funding
32 authors at 9 institutions in 2 countries.
Sabine Ottilie *Department of Pediatrics, University of California, San Diego, Gilman Dr, La Jolla, CA, 92093, USA.
Madeline R Luth *Department of Pediatrics, University of California, San Diego, Gilman Dr, La Jolla, CA, 92093, USA.
Erich Hellemann *Department of Biological Sciences, University of Pittsburgh, 4249 Fifth Avenue, Pittsburgh, PA, 15260, USA.ORCID http://orcid.org/0000-0002-5565-8614
Gregory M Goldgof *Department of Pediatrics, University of California, San Diego, Gilman Dr, La Jolla, CA, 92093, USA.
Eddy VigilDepartment of Pediatrics, University of California, San Diego, Gilman Dr, La Jolla, CA, 92093, USA.
Prianka KumarDepartment of Pediatrics, University of California, San Diego, Gilman Dr, La Jolla, CA, 92093, USA.
Andrea L CheungDepartment of Pediatrics, University of California, San Diego, Gilman Dr, La Jolla, CA, 92093, USA.
Miranda SongDepartment of Pediatrics, University of California, San Diego, Gilman Dr, La Jolla, CA, 92093, USA.ORCID http://orcid.org/0000-0003-4448-277X
Karla P Godinez-MaciasDepartment of Pediatrics, University of California, San Diego, Gilman Dr, La Jolla, CA, 92093, USA.
Krypton CarolinoDepartment of Pediatrics, University of California, San Diego, Gilman Dr, La Jolla, CA, 92093, USA.
Jennifer YangDepartment of Pediatrics, University of California, San Diego, Gilman Dr, La Jolla, CA, 92093, USA.
Gisel LopezDepartment of Pediatrics, University of California, San Diego, Gilman Dr, La Jolla, CA, 92093, USA.
Matthew AbrahamDepartment of Pediatrics, University of California, San Diego, Gilman Dr, La Jolla, CA, 92093, USA.
Maureen TarsioDepartment of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York, NY, 13210, USA.
Emmanuelle LeBlancDepartment of Molecular Genetics, University of Toronto, Toronto, ON, M5G 1M1, Canada.ORCID http://orcid.org/0000-0003-4278-0984
Luke WhitesellDepartment of Molecular Genetics, University of Toronto, Toronto, ON, M5G 1M1, Canada.ORCID http://orcid.org/0000-0001-6445-5766
Jake SchenkenDepartment of Pediatrics, University of California, San Diego, Gilman Dr, La Jolla, CA, 92093, USA.
Felicia GunawanDepartment of Pediatrics, University of California, San Diego, Gilman Dr, La Jolla, CA, 92093, USA.
Reysha PatelDepartment of Pediatrics, University of California, San Diego, Gilman Dr, La Jolla, CA, 92093, USA.
Joshua SmithDepartment of Pediatrics, University of California, San Diego, Gilman Dr, La Jolla, CA, 92093, USA.
Melissa S LoveCalibr, a division of The Scripps Research Institutes, La Jolla, CA, 92037, USA.
Roy M WilliamsDepartment of Pediatrics, University of California, San Diego, Gilman Dr, La Jolla, CA, 92093, USA.
Case W McNamaraCalibr, a division of The Scripps Research Institutes, La Jolla, CA, 92037, USA.
William H GerwickCenter for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, La Jolla, CA, 92037, USA.ORCID http://orcid.org/0000-0003-1403-4458
Trey IdekerDepartment of Medicine, University of California San Diego, La Jolla, CA, USA.
Yo SuzukiDepartment of Synthetic Biology and Bioenergy, J. Craig Venter Institute, La Jolla, CA, 92037, USA.
Dyann F WirthDepartment of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA, USA.
Amanda K LukensInfectious Disease and Microbiome Program, Broad Institute, Cambridge, MA, 02142, USA.
Patricia M KaneDepartment of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York, NY, 13210, USA.
Leah E CowenDepartment of Molecular Genetics, University of Toronto, Toronto, ON, M5G 1M1, Canada.ORCID http://orcid.org/0000-0001-5797-0110
Jacob D DurrantDepartment of Biological Sciences, University of Pittsburgh, 4249 Fifth Avenue, Pittsburgh, PA, 15260, USA.
Elizabeth A WinzelerDepartment of Pediatrics, University of California, San Diego, Gilman Dr, La Jolla, CA, 92093, USA. ewinzeler@health.ucsd.edu.ORCID http://orcid.org/0000-0002-4049-2113
University of California San Diego · USUniversity of Toronto · CABroad Institute · USScripps Research Institute · USSUNY Upstate Medical University · USUniversity of Pittsburgh · USJ. Craig Venter Institute · USScripps Institution of Oceanography · USSociety for Neuroscience · US
Funding
SYNTHETIC SYSTEMSP50GM085764 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI WINZELER, ELIZABETH A · 2010 to 2018
$23.3MTraining Program in Basic Clinical GeneticsT32GM008666 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HAMILTON, BRUCE A · 1998 to 2021
$8.6MTargeting Hsp90 in cryptococcal fungal pathogenesisR01AI120958 · NIAID · UNIVERSITY OF TORONTO · PI BROWN, LAUREN ELAINE, COWEN, LEAH ELIZABETH · 2016 to 2019
$3.2MMechanisms of RAVE-induced V-ATPase assemblyR01GM127364 · NIGMS · UPSTATE MEDICAL UNIVERSITY · PI KANE, PATRICIA M · 2018 to 2021
$1.2MBill and Melinda Gates Foundation (Bill & Melinda Gates Foundation) OPP1054480Bill and Melinda Gates Foundation (Bill & Melinda Gates Foundation) OPP1107194Bill and Melinda Gates Foundation (Bill & Melinda Gates Foundation) OPP1141300Bill and Melinda Gates Foundation (Bill & Melinda Gates Foundation) OPP1171497NIAID NIH HHS R01 AI120958NIGMS NIH HHS P50 GM085764NIGMS NIH HHS R01 GM127364NIGMS NIH HHS T32 GM008666U.S. Department of Health & Human Services | National Institutes of Health (NIH) GM085764U.S. Department of Health & Human Services | National Institutes of Health (NIH) GM127364U.S. Department of Health & Human Services | National Institutes of Health (NIH) R01AI120958-01A1U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) GM008666
Abstract
In vitro evolution and whole genome analysis were used to comprehensively identify the genetic determinants of chemical resistance in Saccharomyces cerevisiae. Sequence analysis identified many genes contributing to the resistance phenotype as well as numerous amino acids in potential targets that may play a role in compound binding. Our work shows that compound-target pairs can be conserved across multiple species. The set of 25 most frequently mutated genes was enriched for transcription factors, and for almost 25 percent of the compounds, resistance was mediated by one of 100 independently derived, gain-of-function SNVs found in a 170 amino acid domain in the two Zn
Indexed as
Saccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsGene Expression Regulation, FungalTranscription FactorsXenobioticsSaccharomyces cerevisiae ProteinsTranscription FactorsXenobiotics
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