Evidence map›Paper›PMID 35149753›Full record

ArticleCommunications biology2022

Three-dimensional spatial transcriptomics uncovers cell type localizations in the human rheumatoid arthritis synovium.

Sanja Vickovic, Denis Schapiro, Konstantin Carlberg, Britta Lötstedt, Ludvig Larsson, Franziska Hildebrandt, Marina Korotkova, Aase H Hensvold, Anca I Catrina, Peter K Sorger and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.

0numbers the graph read from it
0cells of the map it votes in
58citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

58 citing papers in PubMed.

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  18. Spatial transcriptomic applications in orthopedics.Connective tissue research · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Sanja VickovicKlarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA. vickovic@broadinstitute.org.ORCID http://orcid.org/0000-0003-0985-9885
Denis Schapiro *Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-9391-5722
Konstantin Carlberg *Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden.ORCID http://orcid.org/0000-0002-1774-6058
Britta Lötstedt *Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Ludvig Larsson *Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden.ORCID http://orcid.org/0000-0003-4209-2911
Franziska HildebrandtDepartment of Molecular Biosciences, the Wenner Gren Institute, Stockholm University, Stockholm, Sweden.
Marina KorotkovaKarolinska Institutet, Division of Rheumatology, Department of Medicine, Center for Molecular Medicine, Stockholm, Sweden.
Aase H HensvoldKarolinska Institutet, Division of Rheumatology, Department of Medicine, Center for Molecular Medicine, Stockholm, Sweden.
Anca I CatrinaKarolinska Institutet, Division of Rheumatology, Department of Medicine, Center for Molecular Medicine, Stockholm, Sweden.
Peter K SorgerLaboratory of Systems Pharmacology, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0000-0002-3364-1838
Vivianne MalmströmKarolinska Institutet, Division of Rheumatology, Department of Medicine, Center for Molecular Medicine, Stockholm, Sweden.ORCID http://orcid.org/0000-0001-9251-8082
Aviv RegevKlarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0003-3293-3158
Patrik L StåhlScience for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden.ORCID http://orcid.org/0000-0002-2207-7370

Funding

Systems Pharmacology of Therapeutic and Adverse Responses to ImmuneCheckpoint and Small Molecule DrugsU54CA225088 · NCI · HARVARD MEDICAL SCHOOL · PI SORGER, PETER KARL · 2018 to 2022
$10.8M
Howard Hughes Medical InstituteKnut och Alice Wallenbergs Stiftelse (Knut and Alice Wallenberg Foundation) 2017.0454NCI NIH HHS U54 CA225088Royal Swedish Academy of Sciences (Kungl. Vetenskapsakademien) BS2019-0040Svenska Sällskapet för Medicinsk Forskning (Swedish Society for Medical Research) P19-0052
6 · The paper itself

Abstract

The inflamed rheumatic joint is a highly heterogeneous and complex tissue with dynamic recruitment and expansion of multiple cell types that interact in multifaceted ways within a localized area. Rheumatoid arthritis synovium has primarily been studied either by immunostaining or by molecular profiling after tissue homogenization. Here, we use Spatial Transcriptomics, where tissue-resident RNA is spatially labeled in situ with barcodes in a transcriptome-wide fashion, to study local tissue interactions at the site of chronic synovial inflammation. We report comprehensive spatial RNA-Seq data coupled to cell type-specific localization patterns at and around organized structures of infiltrating leukocyte cells in the synovium. Combining morphological features and high-throughput spatially resolved transcriptomics may be able to provide higher statistical power and more insights into monitoring disease severity and treatment-specific responses in seropositive and seronegative rheumatoid arthritis.

Indexed as

Arthritis, RheumatoidTranscriptomeHumansSynovial Membrane

Identifiers

PMID35149753
PMCPMC8837632

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.