ArticleStem cell reports2022
Expanding homogeneous culture of human primordial germ cell-like cells maintaining germline features without serum or feeder layers.
Article in Stem cell reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- One-Step Induction of Oogonia-Like and Pro-Spermatogonia-Like Cells From Porcine Pluripotent Stem Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Integrated metabolomic and transcriptomic analyses reveal that glutamine promotes chicken primordial germ cell‑like cell specification potentially through α-ketoglutarate.Animal bioscience · 2026Article
- Article
- Endocrinology at a Miniature Level: Pluripotent Stem-Cell-Derived Organoid Models of Hypothalamus-Pituitary Axes.Biomolecules · 2026Review
- Reconstitution of the cellular niche requirements for primordial germ cell-like cell progression in humans.Stem cell reports · 2026Article
- Branched Schrödinger Bridge Matching.ArXiv · 2026Article
- The road to restore male fertility using in vitro-derived germ cells.Human reproduction update · 2026Review
- Transitioning from human primordial germ cells to embryonic germ cells.Stem cell reports · 2026Article
- High resolution multi-scale profiling of embryonic germ cell-like cell derivation reveals pluripotent state transitions in humans.Stem cell reports · 2026Article
- Driving precision medicine in testicular germ cell tumors: from preclinical models to clinical applications.Therapeutic advances in medical oncology · 2026Review
- In Vitro Gamete Production in Mammals: Decades of Pioneering Approaches in Germ Cell and Stem Cell Biology Supporting Innovative Discovery and Expansion of Human Reproductive Potential.Molecular reproduction and development · 2025Review
- Entangled Schrödinger Bridge Matching.ArXiv · 2025Article
- Building a human pluripotent stem cell-based gonadal niche: improving in vitro systems with in vivo insights.Human reproduction update · 2025Review
- Establishment of human gastrulating stem cells with the capacity of stable differentiation into multiple gastrulating cell types.Cell research · 2025Article
- Modeling the extension of ovarian function after therapeutic targeting of the primordial follicle reserve.Human reproduction update · 2025Review
- A Polyomavirus-Positive Merkel Cell Carcinoma Mouse Model Supports a Unified Origin for Somatic and Germ Cell Cancers.Cancers · 2025Article
- Advances inFrontiers in endocrinology · 2025Review
- Generating human primordial germ cell-like cells from pluripotent stem cells: a scoping review ofHuman reproduction open · 2025Review
- Association Between Activated Loci of HML-2 Primate-Specific Endogenous Retrovirus and Newly Formed Chromatin Contacts in Human Primordial Germ Cell-like Cells.International journal of molecular sciences · 2024Article
- Article
Corrections and comments
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Authors and funding
16 authors.
Funding
Abstract
In vitro expansion of human primordial germ cell-like cells (hPGCLCs), a pluripotent stem cell-derived PGC model, has proved challenging due to rapid loss of primordial germ cell (PGC)-like identity and limited cell survival/proliferation. Here, we describe long-term culture hPGCLCs (LTC-hPGCLCs), which actively proliferate in a serum-free, feeder-free condition without apparent limit as highly homogeneous diploid cell populations maintaining transcriptomic and epigenomic characteristics of hPGCLCs. Histone proteomics confirmed reduced H3K9me2 and increased H3K27me3 marks in LTC-hPGCLCs compared with induced pluripotent stem cells (iPSCs). LTC-hPGCLCs established from multiple human iPSC clones of both sexes were telomerase positive, senescence-free cells readily passaged with minimal cell death or deviation from the PGC-like identity. LTC-hPGCLCs are capable of differentiating to DAZL-positive M-spermatogonia-like cells in the xenogeneic reconstituted testis (xrTestis) organ culture milieu as well as efficiently producing fully pluripotent embryonic germ cell-like cells in the presence of stem cell factor and fibroblast growth factor 2. Thus, LTC-hPGCLCs provide convenient access to unlimited amounts of high-quality and homogeneous hPGCLCs.
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