Evidence map›Paper›PMID 35147955›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Assays for Apical Constriction Using the Xenopus Model.

Austin T Baldwin, Ivan K Popov, John B Wallingford, Chenbei Chang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.0field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Austin T BaldwinDepartment of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA.
Ivan K PopovDepartment of Cell, Developmental and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL, USA.
John B WallingfordDepartment of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA. wallingford@austin.utexas.edu.
Chenbei ChangDepartment of Cell, Developmental and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL, USA. cchang@uab.edu.
The University of Texas at Austin · USUniversity of Alabama at Birmingham · US

Funding

Molecular and cell biological basis of convergent extensionR01HD099191 · NICHD · UNIVERSITY OF TEXAS AT AUSTIN · PI John B Wallingford · 2020 to 2026
$2.7M
Regulation of apical constriction of bottle cells by the RhoGEF protein Plekhg5 during gastrulation morphogenesisR01GM127371 · NIGMS · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI CHANG, CHENBEI · 2019 to 2022
$1.5M
Dynamic analysis of apical constriction activity during neural tube closureF32HD094521 · NICHD · UNIVERSITY OF TEXAS AT AUSTIN · PI BALDWIN, AUSTIN · 2018 to 2020
$162k
NICHD NIH HHS F32 HD094521NICHD NIH HHS R01 HD099191NIGMS NIH HHS R01 GM127371
6 · The paper itself

Abstract

Apical constriction refers to the active, actomyosin-driven process that reduces apical cell surface area in epithelial cells. Apical constriction is utilized in epithelial morphogenesis during embryonic development in multiple contexts, such as gastrulation, neural tube closure, and organogenesis. Defects in apical constriction can result in congenital birth defects, yet our understanding of the molecular control of apical constriction is relatively limited. To uncover new genetic regulators of apical constriction and gain mechanistic insight into the cell biology of this process, we need reliable assay systems that allow real-time observation and quantification of apical constriction as it occurs and permit gain- and loss-of-function analyses to explore gene function and interaction during apical constriction. In this chapter, we describe using the early Xenopus embryo as an assay system to investigate molecular mechanisms involved in apical constriction during both gastrulation and neurulation.

Indexed as

GastrulationNeurulationAnimalsConstrictionMorphogenesisXenopus laevisActinApical constrictionBottle cellMyosinNeural tube closureXenopus

Identifiers

PMID35147955
PMCPMC10695293
OpenAlexW4210970696

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.