Evidence map›Paper›PMID 35147953›Full record

ReviewMethods in molecular biology (Clifton, N.J.)2022

Imaging Epidermal Cell Rearrangement in the C. elegans Embryo.

Jeff Hardin, Joel Serre, Ryan King, Elise Walck-Shannon, David Reiner

Open access · greenAbstract readReview
In one paragraph

Review in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.2field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Temporal Analysis of Embryonic Epidermal Morphogenesis inInternational journal of molecular sciences · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 1 country.

Jeff HardinDepartment of Integrative Biology, University of Wisconsin-Madison, Madison, WI, USA. jdhardin@wisc.edu.
Joel SerreProgram in Genetics, University of Wisconsin-Madison, Madison, WI, USA.
Ryan KingDepartment of Biology, St. Norbert College, De Pere, WI, USA.
Elise Walck-ShannonDepartment of Biology, Washington University in St. Louis, St. Louis, MO, USA.
David ReinerTexas A&M Health Science Center, Houston, TX, USA.
University of Wisconsin–Madison · USSt. Norbert College · USTexas A&M Health Science Center · USWashington University in St. Louis · US

Funding

PREDOCTORAL TRAINING PROGRAM IN GENETICST32GM007133 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI PERNA, NICOLE T · 1985 to 2023
$16.5M
Tension-dependent regulation of alpha-catenin during morphogenesis in C. elegansR01GM058038 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI HARDIN, JEFFREY D · 1998 to 2021
$6.2M
Regulation of dynamic actin networks during epithelial morphogenesisR35GM145312 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI Jeffrey D Hardin · 2022 to 2026
$3.0M
Trio/CARMIL Regulation of Epithelial Cell RearrangementR01GM127687 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI HARDIN, JEFFREY D · 2018 to 2021
$1.2M
NIGMS NIH HHS R01 GM058038NIGMS NIH HHS R01 GM127687NIGMS NIH HHS R35 GM145312NIGMS NIH HHS T32 GM007133
6 · The paper itself

Abstract

The Caenorhabditis elegans embryo is well suited for analysis of directed cell rearrangement via modern microscopy, due to its simple organization, short generation time, transparency, invariant lineage, and the ability to generate engineered embryos expressing various fluorescent proteins. This chapter provides an overview of routine microscopy techniques for imaging dorsal intercalation, a convergent extension-like morphogenetic movement in the embryonic epidermis of C. elegans, including making agar mounts, low-cost four-dimensional (4D) Nomarski microscopy, laser microsurgery, and 4D fluorescence microscopy using actin and junctional fusion proteins, as well as tissue-specific promoters useful for studying dorsal intercalation.

Indexed as

Caenorhabditis elegansCaenorhabditis elegans ProteinsAnimalsEmbryo, NonmammalianEpidermal CellsEpidermisMicroscopy, FluorescenceMorphogenesisCaenorhabditis elegans ProteinsC. elegansCell intercalationConvergent extensionEpidermisMorphogenesis

Identifiers

PMID35147953
PMCPMC9528972
OpenAlexW4211023214

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.