Evidence map›Paper›PMID 35147951›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Dissection, Fixation, and Immunostaining of the Drosophila Midgut.

Jia Chen, Daniel St Johnston

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. The utility ofFrontiers in immunology · 2024
    Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Jia ChenThe Gurdon Institute and the Department of Genetics, University of Cambridge, Cambridge, UK.
Daniel St JohnstonThe Gurdon Institute and the Department of Genetics, University of Cambridge, Cambridge, UK. d.stjohnston@gurdon.cam.ac.uk.

Funding

Cancer Research UK A14492Cancer Research UK A24823Wellcome Trust 080007Wellcome Trust 092096Wellcome Trust 203144Wellcome Trust 207496Wellcome Trust 207496/Z/17/Z
6 · The paper itself

Abstract

The Drosophila midgut is mainly composed of highly polarized epithelial cells called enterocytes that establish their apical-basal polarity in a fundamentally different way from other Drosophila epithelia. The roles of polarity factors in the midgut can be studied by generating clones of homozygous mutant cells in the background of wild-type tissue. In this chapter, we will introduce and discuss the procedures for producing positively marked mutant clones in the midgut and describe specific protocols for dissecting, fixing, and immunostaining this tissue.

Indexed as

DrosophilaDrosophila ProteinsAnimalsDigestive SystemDrosophila melanogasterEpithelial CellsEpitheliumDrosophila ProteinsDissectionDrosophila midgutHeat fixationImmunostainingMARCM

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.