ArticleMethods in molecular biology (Clifton, N.J.)2022
Two-Photon Cell and Tissue Level Laser Ablation Methods to Study Morphogenetic Biomechanics.
Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- A brief guide to the in vivo chemogenetic cell ablation approach in zebrafish.Molecules and cells · 2026Article
- Sub-50-femtosecond gain-managed amplified pulses enhance nonlinear ablation efficiency.Biomedical optics express · 2026Article
- Mechanical constraints on cell plasticity: insights from the olfactory epithelium.Frontiers in cell and developmental biology · 2026Review
- A multicellular star-shaped actin network underpins epithelial organization and connectivity.Nature communications · 2025Article
- A lateral tension model for mouse cranial neural tube closure.bioRxiv : the preprint server for biology · 2025Article
- Quantifying mechanical forces during vertebrate morphogenesis.Nature materials · 2024Article
- More than double the fun with two-photon excitation microscopy.Communications biology · 2024Review
- The surface ectoderm exhibits spatially heterogenous tension that correlates with YAP localisation during spinal neural tube closure in mouse embryos.Cells & development · 2023Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Laser ablation is routinely performed to infer mechanical tension in cells and tissues. Here we describe our method of two-photon laser ablation at the cellular and tissue level in mouse embryos. The primary outcome of these experiments is initial retraction following ablation, which correlates with, and so can be taken as a measure of, the tensile stress that structure was under before ablation. Several experimental variables can affect interpretation of ablation tests. Pre-test factors include differences in physical properties such as viscoelasticity between experimental conditions. Factors relevant during the test include viability of the cells at the point of ablation, image acquisition rate and the potential for overzealous ablations to cause air bubbles through heat dissipation. Post-test factors include intensity-biased image registration that can artificially produce apparent directionality. Applied to the closing portion of the mouse spinal neural tube, these methods have demonstrated long-range biomechanical coupling of the embryonic structure and have identified highly contractile cell populations involved in its closure process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.