Evidence map›Paper›PMID 35147838›Full record

ArticleCell and tissue banking2022

Overexpression of sonic hedgehog enhances the osteogenesis in rat ectomesenchymal stem cells.

Weijiang Wu, Zhe Wang, Zhijian Zhang, Wenjing Yang, Xin Fan, Jili Xu, Zhiqiang Huang, Qixiang Shao

Abstract read
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In one paragraph

Article in Cell and tissue banking, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Weijiang WuDepartment of Immunology, Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, People's Republic of China.
Zhe WangDepartment of Histology and Embryology, School of Medicine, Jiangsu University, Zhenjiang, 212001, Jiangsu, People's Republic of China.
Zhijian ZhangDepartment of Histology and Embryology, School of Medicine, Jiangsu University, Zhenjiang, 212001, Jiangsu, People's Republic of China.
Wenjing YangDepartment of Histology and Embryology, School of Medicine, Jiangsu University, Zhenjiang, 212001, Jiangsu, People's Republic of China.
Xin FanDepartment of General Surgery, Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, Jiangsu, People's Republic of China.
Jili XuDepartment of Immunology, Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, People's Republic of China.
Zhiqiang HuangDepartment of Immunology, Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, People's Republic of China.
Qixiang ShaoDepartment of Immunology, Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, People's Republic of China. shao_qx@ujs.edu.cn.
Jiangsu University · CNJiangsu Vocational College of Medicine · CN

Funding

Natural Science Foundation of Jilin Province 81273202Natural Science Foundation of Jilin Province 81671541Natural Science Foundation of Jilin Province 81701545Natural Science Foundation of Jilin Province 82071738
6 · The paper itself

Abstract

Ectoderm-derived mesenchymal stem cells (EMSCs) were used as potential seed cells for bone tissue engineering to treat bone defects due to their capability of rapid proliferation and osteogenic differentiation. Sonic hedgehog (Shh) signaling was reported to play an important role in the development of bone tissue, but its role is not understood. The present study investigated the role of Shh molecule in osteogenic differentiation of rat EMSCs in vitro. Rat EMSCs were isolated form nasal respiratory mucosa and identified with immunofluorescence and analyzed with other methods, including reverse transcriptase polymerase chain reaction (qPCR) and western blotting. EMSCs expressed CD90, CD105, nestin, and vimentin. On the seventh day of osteogenic induction, expression levels of Shh and Gli1 was higher according to the result of qPCR and Western blotting. After induction for 14 days, higher alkaline phosphatase (ALP) activity and more mineralized nodules were seen in comparison to the cells that did not undergo induction. Shh signaling appears to enhance osteogenic differentiation of rat EMSCs, suggesting that Shh signaling directs the lineage differentiation of ectodermal stem cells and represents a promising strategy for skeletal tissue regeneration.

Indexed as

Mesenchymal Stem CellsOsteogenesisAlkaline PhosphataseAnimalsCell DifferentiationCells, CulturedHedgehog ProteinsRatsAlkaline PhosphataseHedgehog ProteinsShh protein, ratAlkaline phosphataseEctomesenchymal stem cellsGli1OsteogenesisSonic hedgehog

Identifiers

PMID35147838
OpenAlexW4211257604

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.