Evidence map›Paper›PMID 35146457›Full record

ArticleF&S science2021

Fresh and cryopreserved ovarian tissue from deceased young donors yields viable follicles.

Yuting Fan, Colleen L Flanagan, Margaret A Brunette, Andrea S Jones, Brendon M Baker, Sherman J Silber, Ariella Shikanov

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in F&S science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Review
  6. DynamicFrontiers in endocrinology · 2022
    Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yuting FanDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Colleen L FlanaganDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Margaret A BrunetteDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Andrea S JonesDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Brendon M BakerDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Sherman J SilberInfertility Center of St Louis, St Luke's Hospital, St, Louis, MO 463017, USA.
Ariella ShikanovDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.

Funding

Tissue Engineering and RegenerationT32DE007057 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DAVID H. KOHN · 1985 to 2026
$17.3M
Career Training in Reproductive BiologyT32HD079342 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Suzanne M MOENTER · 2014 to 2026
$2.3M
Engineering an immuno-isolating hydrogel for restoring ovarian endocrine functionR01EB022033 · NIBIB · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SHIKANOV, ARIELLA · 2016 to 2019
$1.5M
NIBIB NIH HHS R01 EB022033NICHD NIH HHS T32 HD079342NIDCR NIH HHS T32 DE007057
6 · The paper itself

Abstract

objectiveOvarian tissue cryopreservation is one of the crucial options for fertility preservation. Transplantation of cryopreserved ovarian tissue was proven to restore ovarian endocrine function in patients with premature ovarian insufficiency. Ovaries from deceased donors potentially serve as an excellent and readily available tissue for the translational and basic research. In this study, we used ovaries obtained from 5 deceased donors aged 18-26 years, to evaluate the number and quality of ovarian follicles isolated before and after cryopreservation.

designPreclinical.

settingAcademic biomedical research laboratory. PATIENTS: De-identified deceased human donors.

interventionsSlow-freeze cryopreservation and thawing.

main outcome measuresFollicle count, follicle density, follicle viability using immunohistochemical staining (TUNEL).

resultsThe follicle density negatively correlated with age in both cryopreserved/thawed and fresh group. A total of 2803 follicles from fresh and 1608 follicles from cryopreserved tissues were classified and analyzed using Hematoxylin and eosin staining. There was no significant difference in the percent of morphologically normal follicles between two groups. TUNEL assay indicated no higher DNA damage in the follicles and the stroma cells after cryopreservation. Morphologically normal preantral follicles were enzymatically isolated from both fresh and cryopreserved tissue with 88.51 ± 5.93% (mean ± SD) of the isolated follicles confirmed viable using LIVE/DEAD evaluation.

conclusionsOur results indicate the ovarian tissue from deceased donors maintain high quality after long time extracorporeal circulation and transportation from the hospital to the laboratory. High survival rate of follicles at different developmental stages suggested tolerance to the cryopreservation process. Human ovarian tissues obtained from deceased donors is an ample source tissue and can be applied to promoting research and future clinical applications.

Indexed as

Fertility PreservationOvaryCryopreservationFemaleFreezingHumansOvarian Follicleartificial ovarydeceased donor tissuefollicle isolationhuman follicleovarian tissue cryopreservation

Identifiers

PMID35146457
PMCPMC8823279

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.