Evidence map›Paper›PMID 35146446›Full record

ArticleSTAR protocols2022

Detecting microRNA-mediated gene regulatory effects in murine neuronal subpopulations.

Neal D Amin, Gokhan Senturk, Marito Hayashi, Shawn P Driscoll, Samuel L Pfaff

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Neal D AminGene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines, La Jolla, CA 92037, USA.
Gokhan SenturkGene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines, La Jolla, CA 92037, USA.
Marito HayashiGene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines, La Jolla, CA 92037, USA.
Shawn P DriscollGene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines, La Jolla, CA 92037, USA.
Samuel L PfaffGene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines, La Jolla, CA 92037, USA.
Salk Institute for Biological Studies · US

Funding

A BIOBEHAVIORAL RESEARCH TRAINING PROGRAMT32MH019938 · NIMH · STANFORD UNIVERSITY · PI Corey J Keller, RACHEL MANBER · 1994 to 2026
$9.4M
Characterization of spinal circuits underlying motor synergy functionR01NS123160 · NINDS · SALK INSTITUTE FOR BIOLOGICAL STUDIES · PI PFAFF, SAMUEL L. · 2021 to 2025
$3.0M
BD FACSAria Fusion for Flow Cytometry Core FacilityS10OD023689 · OD · SALK INSTITUTE FOR BIOLOGICAL STUDIES · PI ZHENG, YE · 2018 to 2018
$530k
MiR-218 regulatory networks in adult mice and its relationship to ALSR21NS121846 · NINDS · SALK INSTITUTE FOR BIOLOGICAL STUDIES · PI PFAFF, SAMUEL L. · 2021 to 2021
$529k
Tools for regulated expression control of miR-218R03NS121480 · NINDS · SALK INSTITUTE FOR BIOLOGICAL STUDIES · PI PFAFF, SAMUEL L. · 2021 to 2021
$192k
NIH HHS S10 OD023689NIMH NIH HHS T32 MH019938NINDS NIH HHS R01 NS123160NINDS NIH HHS R03 NS121480NINDS NIH HHS R21 NS121846
6 · The paper itself

Abstract

microRNAs (miRNAs) have unique gene regulatory effects in different neuronal subpopulations. Here, we describe a protocol to identify neuronal subtype-specific effects of a miRNA in murine motor neuron subpopulations. We detail the preparation of primary mouse spinal tissue for single cell RNA sequencing and bioinformatics analyses of pseudobulk expression data. This protocol applies differential gene expression testing approaches to identify miRNA target networks in heterogeneous neuronal subpopulations that cannot otherwise be captured by bulk RNA sequencing approaches. For complete details on the use and execution of this protocol, please refer to Amin et al. (2021).

Indexed as

MicroRNAsAnimalsComputational BiologyGene Expression RegulationMiceNeuronsSequence Analysis, RNAMicroRNAsBioinformaticsCell isolationDevelopmental biologyFlow Cytometry/Mass CytometryGene ExpressionModel OrganismsNeuroscienceSingle Cell

Identifiers

PMID35146446
PMCPMC8801384
OpenAlexW4206951853

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.