ArticleParasite epidemiology and control2022
Amplicon-based next-generation sequencing of eukaryotic nuclear ribosomal genes (metabarcoding) for the detection of single-celled parasites in human faecal samples.
Article in Parasite epidemiology and control, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 41 citations in OpenAlex.
- Intraspecies genetic diversity and host-associated distribution of Endolimax nana in Sumba Island, Indonesia.Tropical medicine and health · 2026Article
- Detection of Echinococcus multilocularis in coyotes in Washington State, USA highlights need for increased wildlife surveillance.PLoS neglected tropical diseases · 2026Article
- Next-Gen intestinal parasite detection: Leveraging metataxonomics for improved diagnosis of intestinal protists and helminths.PloS one · 2026Article
- Article
- Aspects of Genetic Diversity, Host Specificity and Public Health Significance of Single-Celled Intestinal Parasites Commonly Observed in Humans and Mostly Referred to as 'Non-Pathogenic'.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2025Review
- Characterizing the Helminth Community of the Mountain Gazelle (Gazella gazella Pallas, 1766) Through DNA Metabarcoding.Acta parasitologica · 2025Article
- Structure analysis of human gut microbiota associated with single-celled gut protists using Next-Generation Sequencing of 16S and 18S rRNA genes.Computational and structural biotechnology journal · 2025Article
- DNA Barcoding Using 18S rRNA Gene Fragments for Identification of Tick-Borne Protists in Ticks in the Republic of Korea.Pathogens (Basel, Switzerland) · 2024Article
- Optimization of 18 S rRNA metabarcoding for the simultaneous diagnosis of intestinal parasites.Scientific reports · 2024Article
- Climate influences the gut eukaryome of wild rodents in the Great Rift Valley of Jordan.Parasites & vectors · 2024Article
- Metabarcoding study of potential pathogens and zoonotic risks associated with dog feces in Seoul, South Korea.PLoS neglected tropical diseases · 2024Article
- Development of nucleic acid lateral flow immunoassay for molecular detection of Entamoeba moshkovskii and Entamoeba dispar in stool samples.Scientific reports · 2024Article
- Detection of zoonoticParasite epidemiology and control · 2024Article
- Monitoring potentially pathogenic protists in sewage sludge using Metataxonomics.Food and waterborne parasitology · 2023Article
- An overview of the trypanosomatid (Kinetoplastida: Trypanosomatidae) parasites infecting several mammal species in Colombia.Parasites & vectors · 2022Article
- Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Comprehensive detection and differentiation of intestinal protists mostly rely on DNA-based methods. Here, we evaluated next-generation sequencing of eukaryotic nuclear ribosomal genes (metabarcoding) for the detection and differentiation of intestinal eukaryotic protists in the stool of healthy Tunisian individuals. Thirty-six faecal DNA samples previously evaluated by microscopy and ameboid species-specific PCRs were tested. The hypervariable regions V3-V4 and V3-V5 of the 18S rRNA gene were amplified using three universal eukaryotic primer sets and sequenced using Illumina®MiSeq sequencing. In addition, real-time PCR assays were used to detect
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