ArticleNature methods2022
A genetically encoded sensor for in vivo imaging of orexin neuropeptides.
Article in Nature methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- Optical insights into spatial precision and release heterogeneity of neuromodulatory transmission.iScience · 2026Article
- Multifiber Array-Based Photometry System for Multiregional Functional Mapping in the Mouse Brain.The European journal of neuroscience · 2026Article
- Next-generation multicolor indicators for in vivo imaging of norepinephrine.Nature methods · 2026Article
- Empathy and prosocial behavior powered by orexin-driven theta oscillations.Science (New York, N.Y.) · 2026Article
- Development of a genetically encoded melanocortin sensor for high sensitivity in vivo imaging.Molecular metabolism · 2025Article
- Orexinergic projections to substantia innominata mediate arousal and analgesia.British journal of anaesthesia · 2025Article
- Orexin population activity precisely reflects net body movement across behavioral and metabolic states.eLife · 2025Article
- In vivo measurement of an Apelin gradient with a genetically encoded APLNR conformation biosensor.Nature communications · 2025Article
- Genetically encoded sensors illuminateIBRO neuroscience reports · 2025Article
- Mapping and decoding neuropeptide signaling networks in nervous system function.Current opinion in neurobiology · 2025Review
- An integrated microfluidic and fluorescence platform for probing in vivo neuropharmacology.Neuron · 2025Article
- Comparative Perspectives on Neuropeptide Function and Social Isolation.Biological psychiatry · 2025Review
- A high-performance GRAB sensor reveals differences in the dynamics and molecular regulation between neuropeptide and neurotransmitter release.Nature communications · 2025Article
- Live imaging of paracrine signaling: Advances in visualization and tracking techniques.Cell structure and function · 2025Review
- Hypocretin in the nucleus accumbens shell modulates social approach in female but not male California mice.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2024Article
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Neuropeptide signaling network of Caenorhabditis elegans: from structure to behavior.Genetics · 2024Review
- Contribution of hypothalamic orexin (hypocretin) circuits to pathologies of motivation.British journal of pharmacology · 2024Review
- Neuromodulator and neuropeptide sensors and probes for precise circuit interrogation in vivo.Science (New York, N.Y.) · 2024Review
- Applications of Lightsheet Fluorescence Microscopy by High Numerical Aperture Detection Lens.The journal of physical chemistry. B · 2024Review
Corrections and comments
- Erratum issued
Authors and funding
21 authors.
Funding
Abstract
Orexins (also called hypocretins) are hypothalamic neuropeptides that carry out essential functions in the central nervous system; however, little is known about their release and range of action in vivo owing to the limited resolution of current detection technologies. Here we developed a genetically encoded orexin sensor (OxLight1) based on the engineering of circularly permutated green fluorescent protein into the human type-2 orexin receptor. In mice OxLight1 detects optogenetically evoked release of endogenous orexins in vivo with high sensitivity. Photometry recordings of OxLight1 in mice show rapid orexin release associated with spontaneous running behavior, acute stress and sleep-to-wake transitions in different brain areas. Moreover, two-photon imaging of OxLight1 reveals orexin release in layer 2/3 of the mouse somatosensory cortex during emergence from anesthesia. Thus, OxLight1 enables sensitive and direct optical detection of orexin neuropeptides with high spatiotemporal resolution in living animals.
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