ArticleDevelopment (Cambridge, England)2022
Long-term imaging of living adult zebrafish.
Article in Development (Cambridge, England), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- Endothelial Cell-Pericyte Crosstalk in the Brain Balances Microvascular Stability and Plasticity.Arteriosclerosis, thrombosis, and vascular biology · 2026Review
- Ex vivo imaging reveals neutrophil behaviors in the adult zebrafish heart.Journal of cell science · 2026Article
- Longitudinal in vivo imaging of dynamic immune-vascular responses to meningeal cerebrovascular injury.Journal of neuroinflammation · 2026Article
- Quantum Dot-Based Approaches for Labeling Epithelial Damage in Living and Fixed Zebrafish Embryos.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Specialized gas-exchange endothelium of the zebrafish gill.bioRxiv : the preprint server for biology · 2025Article
- Live longitudinal imaging of meningeal cerebrovascular injury and its sequelae in adult zebrafish.bioRxiv : the preprint server for biology · 2025Article
- Non-invasive longitudinal imaging reveals aging-associated changes in neuroimmune cells in adult zebrafish.bioRxiv : the preprint server for biology · 2025Article
- Dynamic cell fate plasticity and tissue reintegration drive functional adult synovial joint regeneration after complete resection.Nature communications · 2025Article
- Zebrafish as a Model Organism for Post-Traumatic Stress Disorder: Insights into Stress Mechanisms and Behavioral Assays.Biology · 2025Review
- Zebrafish Models of Induced Lymphangiogenesis: Current Advancements and Therapeutic Discovery.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Cerebrovascular ageing: how zebrafish can contribute to solving the puzzle.Frontiers in physiology · 2025Article
- Preclinical Implementation of matRadiomics: A Case Study for Early Malformation Prediction in Zebrafish Model.Journal of imaging · 2024Article
- Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy.Biomedical optics express · 2024Article
- A practical guide to light-sheet microscopy for nanoscale imaging: Looking beyond the cell.Journal of applied physics · 2024Article
- Article
- Dermal Dive: An Overview of Cutaneous Wounding Techniques in Zebrafish.The Journal of investigative dermatology · 2024Review
- Macrophages in tissue repair and regeneration: insights from zebrafish.Cell regeneration (London, England) · 2024Review
- Live Imaging of Cutaneous Wound Healing after Rotary Tool Injury in Zebrafish.The Journal of investigative dermatology · 2024Article
- Progress in characterizing ABC multidrug transporters in zebrafish.Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy · 2024Article
- Interwoven processes in fish development: microbial community succession and immune maturation.PeerJ · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The zebrafish has become a widely used animal model due, in large part, to its accessibility to and usefulness for high-resolution optical imaging. Although zebrafish research has historically focused mostly on early development, in recent years the fish has increasingly been used to study regeneration, cancer metastasis, behavior and other processes taking place in juvenile and adult animals. However, imaging of live adult zebrafish is extremely challenging, with survival of adult fish limited to a few tens of minutes using standard imaging methods developed for zebrafish embryos and larvae. Here, we describe a new method for imaging intubated adult zebrafish using a specially designed 3D printed chamber for long-term imaging of adult zebrafish on inverted microscope systems. We demonstrate the utility of this new system by nearly day-long observation of neutrophil recruitment to a wound area in living double-transgenic adult casper zebrafish with fluorescently labeled neutrophils and lymphatic vessels, as well as intubating and imaging the same fish repeatedly. We also show that Mexican cavefish can be intubated and imaged in the same way, demonstrating this method can be used for long-term imaging of adult animals from diverse aquatic species.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.