Evidence map›Paper›PMID 35141626›Full record

ArticleNorth American Spine Society journal2021

Paraspinal muscle degeneration and regenerative potential in a Murine model of Lumbar Disc Injury.

Michael R Davies, Gurbani Kaur, Xuhui Liu, Francisco Gomez Alvarado, Prashant Nuthalapati, Mengyao Liu, Agustin Diaz, Jeffrey C Lotz, Jeannie F Bailey, Brian T Feeley

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in North American Spine Society journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Michael R DaviesUniversity of California, San Francisco, Department of Orthopaedic Surgery, 1700 Owens Street, San Francisco, CA 94158, USA.
Gurbani KaurUniversity of California, San Francisco, Department of Orthopaedic Surgery, 1700 Owens Street, San Francisco, CA 94158, USA.
Xuhui LiuUniversity of California, San Francisco, Department of Orthopaedic Surgery, 1700 Owens Street, San Francisco, CA 94158, USA.
Francisco Gomez AlvaradoUniversity of California, San Francisco, Department of Orthopaedic Surgery, 1700 Owens Street, San Francisco, CA 94158, USA.
Prashant NuthalapatiUniversity of California, San Francisco, Department of Orthopaedic Surgery, 1700 Owens Street, San Francisco, CA 94158, USA.
Mengyao LiuUniversity of California, San Francisco, Department of Orthopaedic Surgery, 1700 Owens Street, San Francisco, CA 94158, USA.
Agustin DiazUniversity of California, San Francisco, Department of Orthopaedic Surgery, 1700 Owens Street, San Francisco, CA 94158, USA.
Jeffrey C LotzUniversity of California, San Francisco, Department of Orthopaedic Surgery, 1700 Owens Street, San Francisco, CA 94158, USA.
Jeannie F BaileyUniversity of California, San Francisco, Department of Orthopaedic Surgery, 1700 Owens Street, San Francisco, CA 94158, USA.
Brian T FeeleyUniversity of California, San Francisco, Department of Orthopaedic Surgery, 1700 Owens Street, San Francisco, CA 94158, USA.
University of California, San Francisco · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

background contextThe association between low back pain and lumbar disc degeneration is not fully characterized. One potentially overlooked factor of this process is the lumbar multifidus, which plays a role in segmental stabilization and locomotion. Previous reports have shown multifidus degeneration to be associated with disc degeneration. The goal of this study was to develop a mouse model of advanced lumbar disc degeneration to recapitulate the pathology of the human multifidus in patients with lumbar disc degeneration and low back pain.

methodsC57BL/6 mice underwent a left anterolateral approach to the lumbar spine and disc puncture with a micro scalpel at L5/6 and L6/S1. Mice underwent behavioral analysis and functional gait testing. A subset of mice underwent 14T T2-weighted MRI to assess disc degeneration and paraspinal muscle quality. At 6 and 15 weeks, mice were sacrificed, and the multifidus muscles were harvested and divided into proximal and distal segments relative to disc injury. Histological analysis was performed to assess muscle degeneration, fiber type, and macrophage density. Fibroadipogenic progenitors (FAPs) were isolated for gene expression analysis with qPCR.

resultsMRI demonstrated decreased intervertebral disc signal and paraspinal muscle atrophy at 6 weeks, with progressive degeneration and atrophy at 15 weeks. Disc injury resulted in delayed functional recovery and impaired gait. Histology demonstrated progressive multifidus fibrofatty degeneration between 6 and 15 weeks. CD68+ macrophage density was increased at 6 weeks but not 15 weeks. FAPs exhibited increased fibrotic and adipogenic gene expression at 6 weeks compared to sham with less of a difference in gene expression by 15 weeks.

conclusionsWe have developed a mouse model of disc injury-mediated paraspinal muscle degeneration that recapitulates features of degenerative muscle pathology observed in patients with lumbar disc degeneration, and highlights the role of FAPs in mediating fibrofatty muscle degeneration after disc injury.

Indexed as

Fibroadipogenic progenitorsGait analysisMultifidus degenerationMurine model

Identifiers

PMID35141626
PMCPMC8820077
OpenAlexW3156570040

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.