ArticleParasite epidemiology and control2022
Molecular-based assay for genotyping
Article in Parasite epidemiology and control, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed, 12 citations in OpenAlex.
- Comparative evaluation of parasitological, serological, and molecular methods for the diagnosis of cutaneous leishmaniasis in the Garmian area, Kurdistan region of Iraq.Parasite epidemiology and control · 2026Article
- Can archived lesion smears be used to identify Leishmania species in regions with high species diversity?Revista do Instituto de Medicina Tropical de Sao Paulo · 2025Article
- Clinical and laboratory characterization of cutaneous leishmaniasis in Chinese migrant workers returned from Iraq.PLoS neglected tropical diseases · 2024Article
- Highly sensitive nested polymerase chain reaction to improve the detection ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- Molecular Identification of Cutaneous Leishmaniasis Species in the Northcentral Iranian Province of Alborz: Is There a New Focus on Cutaneous Leishmaniasis in the Province?Iranian journal of parasitologyArticle
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cutaneous leishmaniasis (CL) is highly prevalent in southern Iraq and neighboring countries, but is non-endemic to the Kurdistan Region of Iraq, particularly in the Garmian area. This study aimed to investigate the causative agent of CL at the molecular level by amplifying the small subunit (18S) rRNA and internal transcribed spacer 1 (ITS1) region. The present study was conducted from December 2019 to December 2020 at Kalar General Hospital, Kalar, Kurdistan Region, Iraq. Eighty-five clinical specimens were collected selectively from patients with suspected CL lesions via fine needle aspiration. After parasitic genomic DNA was extracted from the removed fluid, PCR and DNA sequencing targeting the 18S rRNA and ITS1 region were performed for molecular detection and species identification. Additionally, for 14 samples, the target bands of amplified DNA fragments for both 18S rRNA and ITS1 were extracted and sequenced via Sanger method using both the directional primers employed in the PCR. Seventy-one (83.53%) of the 85 suspected patients had CL, based on amplification of 18S rRNA and ITS1 via PCR. The sequence analysis revealed that all samples were
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