ArticleFrontiers in microbiology2021
Relative and Quantitative Rhizosphere Microbiome Profiling Results in Distinct Abundance Patterns.
Article in Frontiers in microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A Systematic Review of Soil Properties to Support Mycotoxin Model Development with In-Field Soil Sensing.Sensors (Basel, Switzerland) · 2026Pooled it
- TR4-triggered recruitment of suppressive rhizomicrobiome enhances Fusarium wilt resistance in banana.The New phytologist · 2026Article
- Interaction of Microplastics, Plants, and Rhizosphere: A Critical Review.Molecules (Basel, Switzerland) · 2026Review
- Bacterial Community Expansion and Nutrient Activation Underlie Yield Improvement Following Diazotrophic Inoculant Application in Paddy Soil.Microorganisms · 2026Article
- Root-associated bacterial community dynamics and assembly mechanisms in healthy and root rot-infected soybeans.Frontiers in microbiology · 2026Article
- The metatranscriptomic response of the wheat rhizosphere to drought varies with growth stages.ISME communications · 2026Article
- Development of a quantitative PMA-16S rRNA gene sequencing workflow for absolute abundance measurements of seawater microbial communities.Environmental microbiome · 2025Article
- Ecosystem Multifunctionality Regulated by Soil Microbial Activity and Indicator Taxa Versus Biodiversity for Industrial Solar Facilities on the Qinghai-Tibet Plateau.Microorganisms · 2025Article
- Natural recolonization of the amphibian skin bacterial community following disruption by antibiotics.Proceedings. Biological sciences · 2025Article
- Year-Round Quantification, Structure and Dynamics of Epibacterial Communities From Diverse Macroalgae Reveal a Persistent Core Microbiota and Strong Host Specificities.Environmental microbiology reports · 2025Article
- Microbiome changes in the spongeFrontiers in microbiology · 2025Article
- Metatranscriptomic response of the wheat holobiont to decreasing soil water content.ISME communications · 2023Article
- Absolute quantification of viable bacteria abundances in food by next-generation sequencing: Quantitative NGS of viable microbes.Current research in food science · 2023Article
- Isolation, identification, and significance of salivaryFrontiers in cellular and infection microbiology · 2023Article
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Authors and funding
4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Next-generation sequencing is one of the most popular and cost-effective ways of characterizing microbiome in multiple samples. However, most of the currently available amplicon sequencing approaches are limited, as they result in relative abundance profiles of microbial taxa, which does not represent actual abundance in the environment. Here, we combined amplicon sequencing (16S rRNA gene for bacteria and ITS region for fungi) with real-time quantitative PCR (qPCR) to characterize the rhizosphere microbiome of wheat. We show that changes in the relative abundance of major microbial phyla do not necessarily follow the same pattern as the estimated quantitative abundance. Most of the bacterial phyla linked with the rhizosphere of plants grown in soil with no history of water stress showed enrichment patterns in their estimated absolute abundance, which was in contradiction with the trends observed in the relative abundance data. However, in the case of the fungal groups (except for
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