Evidence map›Paper›PMID 35140689›Full record

ArticleFrontiers in microbiology2021

Oceanic Crustal Fluid Single Cell Genomics Complements Metagenomic and Metatranscriptomic Surveys With Orders of Magnitude Less Sample Volume.

Timothy D'Angelo, Jacqueline Goordial, Nicole J Poulton, Lauren Seyler, Julie A Huber, Ramunas Stepanauskas, Beth N Orcutt

Abstract read
In one paragraph

Article in Frontiers in microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Timothy D'AngeloBigelow Laboratory for Ocean Sciences, East Boothbay, ME, United States.
Jacqueline GoordialBigelow Laboratory for Ocean Sciences, East Boothbay, ME, United States.
Nicole J PoultonBigelow Laboratory for Ocean Sciences, East Boothbay, ME, United States.
Lauren SeylerSchool of Natural Science and Mathematics, Stockton University, Galloway, NJ, United States.
Julie A HuberMarine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, MA, United States.
Ramunas StepanauskasBigelow Laboratory for Ocean Sciences, East Boothbay, ME, United States.
Beth N OrcuttBigelow Laboratory for Ocean Sciences, East Boothbay, ME, United States.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fluids circulating through oceanic crust play important roles in global biogeochemical cycling mediated by their microbial inhabitants, but studying these sites is challenged by sampling logistics and low biomass. Borehole observatories installed at the North Pond study site on the western flank of the Mid-Atlantic Ridge have enabled investigation of the microbial biosphere in cold, oxygenated basaltic oceanic crust. Here we test a methodology that applies redox-sensitive fluorescent molecules for flow cytometric sorting of cells for single cell genomic sequencing from small volumes of low biomass (approximately 10

Indexed as

CORKScrustal fluiddeep biosphereIODPmetatranscriptomicsNorth Pondoceanic crustsingle cell genomics

Identifiers

PMID35140689
PMCPMC8819061

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.