Evidence map›Paper›PMID 35140480›Full record

ArticleInfection and drug resistance2022

Insertion Mutation of

Zhen Zhang, Zhulan Yang, Junfeng Zhen, Xiaohong Xiang, Pu Liao, Jianping Xie

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Article in Infection and drug resistance, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Zhen ZhangDepartment of Clinical Laboratory, Chongqing General Hospital, University of Chinese Academy of Sciences, Chongqing, People's Republic of China.
Zhulan YangDepartment of Clinical Laboratory, Southwest Hospital, Army Medical University, Chongqing, People's Republic of China.
Junfeng ZhenDepartment of Clinical Laboratory, Chongqing General Hospital, University of Chinese Academy of Sciences, Chongqing, People's Republic of China.
Xiaohong XiangSchool of Pharmacy, Chongqing Medical and Pharmaceutical College, Chongqing, People's Republic of China.
Pu LiaoDepartment of Clinical Laboratory, Chongqing General Hospital, University of Chinese Academy of Sciences, Chongqing, People's Republic of China.
Jianping XieInstitute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Three Gorges Eco-Environment and Bioresources, Eco-Environment Key Laboratory of the Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Chongqing, 400715, People's Republic of China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposePhage is a new choice for the treatment of multi-drug-resistant bacteria, and phage resistance is also an issue of concern. SWU1 is a mycobacteriophage, and the mechanism of its resistance remain poorly understood.

methodsThe mutant strains which were stably resistant to SWU1 were screened by transposon mutation library. The stage of phage resistance was observed by transmission electron microscope (TEM). The insertion site of transposon was identified by thermal asymmetric interlaced PCR (TAIL-PCR). The possible relationship between insertion site and phage resistance was verified by gene knockout technique. The fatty acid composition of bacterial cell wall was analyzed by Gas Chromatography-Mass Spectrometer (GC-MS). Through the amplification and sequencing of target genes and gene complement techniques to find the mechanism of SWU1 resistance.

resultsThe transposon mutant M12 which was stably resistant to mycobacteriophage SWU1 was successfully screened. It was confirmed that resistance occurred in the adsorption stage of bacteriophage. It was verified that the insertion site of the transposon was located in the

conclusionWe confirmed that the resistance of M12 to SWU1 was related to the functional inactivation of

Indexed as

insertion mutationmycobacteriophagephage resistance M. smegmatisSWU1

Identifiers

PMID35140480
PMCPMC8818766

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.