ArticleInternational journal of nanomedicine2022
Periodontal Ligament Stem Cell-Derived Small Extracellular Vesicles Embedded in Matrigel Enhance Bone Repair Through the Adenosine Receptor Signaling Pathway.
Article in International journal of nanomedicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.
- Preclinical Evidence for the Use of Oral Mesenchymal Stem Cell-Derived Extracellular Vesicles in Bone Regenerative Therapy: A Systematic Review.Stem cells translational medicine · 2023Pooled it
- hsa-let-7c-3p Restrains the Proliferation-to-Osteogenesis Transition of Human Periodontal Ligament Mesenchymal Stem/Stromal Cells via NT5E.Stem cell reviews and reports · 2026Article
- Biomimetic nanovesicles and nanotechnology for oral and maxillofacial diseases.Discover nano · 2026Review
- Exosome Purification Strategy Influences the Regenerative Efficacy of Dental Pulp Stem Cell-Derived Exosomes in Bone Regeneration.Stem cell reviews and reports · 2026Article
- Exosomes in Bone Generation and Repair: Focusing on Bone Microenvironmental Crosstalk and Engineering Biomaterial Designs.International journal of nanomedicine · 2026Review
- Extracellular vesicles from induced pluripotent stem cell-derived periodontal ligament cells enhance proliferation and migration of periodontal ligament cells for periodontal regeneration.Frontiers in cell and developmental biology · 2026Article
- Review
- Human periodontal ligament stem cells-derived small extracellular vesicles mitigate periodontitis by regulating macrophage pyroptosis.Journal of translational medicine · 2025Article
- [Biological characteristics and translational research of dental stem cells].Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2025Review
- Craniomaxillofacial-Derived MSCs in Congenital Defect Reconstruction.Biomolecules · 2025Review
- Isolation methods of exosomes derived from dental stem cells.International journal of oral science · 2025Review
- Extracellular Vesicles in Periodontitis: Pathogenic Mechanisms and Therapeutic Potential.Journal of inflammation research · 2025Review
- Extracellular Vesicle-Integrated Biomaterials in Bone Tissue Engineering Applications: Current Progress and Future Perspectives.International journal of nanomedicine · 2025Review
- Biomimetic materials: a promising strategy for periodontal tissue engineering and regeneration.Frontiers in bioengineering and biotechnology · 2025Review
- Metformin enhances the therapeutic effects of extracellular vesicles derived from human periodontal ligament stem cells on periodontitis.Scientific reports · 2024Article
- Activating the healing process: three-dimensional culture of stem cells in Matrigel for tissue repair.BMC biotechnology · 2024Article
- A MEK inhibitor arrests the cell cycle of human conjunctival fibroblasts and improves the outcome of glaucoma filtration surgery.Scientific reports · 2024Article
- Dental Stem Cell-Derived Exosomes: A Review of Their Isolation, Classification, Functions, and Mechanisms.Stem cells international · 2024Review
- Clinical Potential of Mesenchymal Stem Cell-Derived Exosomes in Bone Regeneration.Journal of clinical medicine · 2023Review
- Rab27a-mediated extracellular vesicle secretion contributes to osteogenesis in periodontal ligament-bone niche communication.Scientific reports · 2023Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeSmall extracellular vesicles (sEVs) are natural biocarriers for biomolecule transfer between cells and promising therapeutic strategies for bone defect repair. In this study, human periodontal ligament stem cell (PDLSC)-derived sEVs (P-EVs) were immobilized in Matrigel to establish a topical cell-free transplantation strategy for bone repair.
methodsPDLSCs were cultured and P-EVs were isolated from the culture supernatant. In a rat bilateral calvarial defect model, P-EV/Matrigel was plugged into one defect and PBS/Matrigel was applied to the other. Bone repair in vivo was assessed by micro-computed tomography, histomorphometry, and immunohistochemical staining. In vitro, we investigated the effects of P-EVs on the proliferation and migration capabilities of bone marrow mesenchymal stem cells (BMMSCs) and explored the potential mechanism of action.
resultsThe in vivo study showed that P-EV/Matrigel accelerated bone tissue repair by increasing cell infiltration when compared with the control. In vitro, P-EVs enhanced proliferation and migration of BMMSCs via increased phosphorylation of AKT and extracellular signal-regulated kinase 1/2 (ERK1/2). The role of P-EV-induced adenosine receptor signaling in AKT and ERK1/2 phosphorylation was a key mediator during enhanced BMMSC migration.
conclusionThese results are the first to demonstrate that P-EVs accelerated the repair of bone defects, partially through promoting cell proliferation and migration. P-EV/Matrigel, which combines topical EV-implantation and extracellular matrix scaffolds, provides a new cell-free strategy for bone tissue repair.
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