Evidence map›Paper›PMID 35139864›Full record

ArticleJournal of orthopaedic surgery and research2022

Dipeptidyl peptidase-4 is associated with myogenesis in patients with adolescent idiopathic scoliosis possibly via mediation of insulin sensitivity.

Zhicheng Dai, Bingchuan Xue, Leilei Xu, Zhenhua Feng, Zhichong Wu, Yong Qiu, Zezhang Zhu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of orthopaedic surgery and research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed, 1 pooled it
0.6field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 1 synthesis or guideline pooled it, 4 citations in OpenAlex.

  1. Pooled it
  2. Review
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  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Zhicheng DaiDepartment of Spine Surgery, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Zhongshan Road 321, Nanjing, 210008, China.
Bingchuan XueDepartment of Spine Surgery, Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.
Leilei XuDepartment of Spine Surgery, Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.
Zhenhua FengDepartment of Spine Surgery, Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.
Zhichong WuDepartment of Spine Surgery, Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.
Yong QiuDepartment of Spine Surgery, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Zhongshan Road 321, Nanjing, 210008, China.
Zezhang ZhuDepartment of Spine Surgery, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Zhongshan Road 321, Nanjing, 210008, China. zezhangzhu@126.com.
Nanjing Drum Tower Hospital · CNNanjing Medical University · CN

Funding

National Natural Science Foundation of China 81772304National Natural Science Foundation of China 81871747
6 · The paper itself

Abstract

backgroundAbnormal metabolic features have been previously described in adolescent idiopathic scoliosis (AIS) patients. As an important regulator involved in energy metabolism, DPP-4 activity was reported to be remarkably decreased in osteoblasts of AIS patients. To date, there was still a lack of knowledge concerning the role of DPP-4 in the myogenesis of AIS.

methodsCirculation DPP-4 level was assessed in the serum of 80 AIS girls and 50 healthy controls by ELISA. Myoblasts were purified from muscle specimens of AIS patients and LDH controls, and then treated with metabolic effectors including glucose and insulin. CCK-8 assay was used to assess the cell viability and myotube fusion index was calculated to evaluate myogenesis ability. Gene expressions of downstream signals of DPP-4 were evaluated by RT-qPCR and Western blot respectively.

resultsAIS girls had remarkably down-expressed DPP-4 in both serum level (0.76 fold) and tissue (0.68 fold) level. Treatment with metabolic effectors led to significantly increased DPP-4 expression in the control cells, while there was no increase of DPP-4 in AIS cells. CCK-8 assay showed that the proliferation rate of control cells was significantly increased after being treated. Remarkably higher fusion index was also observed in the treated control cells. By contrast, the fusion index and cell proliferation rate were comparable between the treated and the untreated AIS cells.

conclusionsOur study suggested a potential role of DPP-4 in abnormal metabolic condition of AIS patients. Compared with control cells, AIS myoblasts presented obviously impaired sensitivity to the treatment of glucose and insulin. Aberrant DPP-4 expression could lead to impaired insulin sensitivity in myoblasts and further influence the cell viability during myogenesis. The molecular mechanism connecting DPP-4 and insulin-related signaling in AIS is worthy of further investigation.

Indexed as

InsulinAdolescentCase-Control StudiesDipeptidyl Peptidase 4FemaleGene ExpressionGlucoseHumansInsulin ResistanceMuscle DevelopmentOsteoblastsReal-Time Polymerase Chain ReactionScoliosisDipeptidyl Peptidase 4DPP4 protein, humanGlucoseInsulinAdolescent idiopathic scoliosisDipeptidyl peptidase-4Insulin sensitivityMetabolismSignaling

Identifiers

PMID35139864
PMCPMC8827187
OpenAlexW4220964025

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.