ArticleActa veterinaria Scandinavica2022
Assessment of three DNA extraction kits for the absolute quantification of strongyle nematode eggs in faecal samples.
Article in Acta veterinaria Scandinavica, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Ambient temperature storage of individual parasitic nematode larvae for whole genome sequencing.PLoS neglected tropical diseases · 2026Article
- Occurrence of gastrointestinal nematodes in lambs in Norway, as assessed by copromicroscopy and droplet digital polymerase chain reaction.Acta veterinaria Scandinavica · 2024Article
- Faecal metabarcoding provides improved detection and taxonomic resolution for non-invasive monitoring of gastrointestinal nematode parasites in wild moose populations.Parasites & vectors · 2023Article
- Comparative Study of DNA Extraction Methods for the PCR Detection of Intestinal Parasites in Human Stool Samples.Diagnostics (Basel, Switzerland) · 2022Article
- Sensitive and accurate DNA metabarcoding of parasitic helminth mock communities using the mitochondrial rRNA genes.Scientific reports · 2022Article
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Authors and funding
4 authors.
Funding
Abstract
backgroundHaemonchus contortus is one of the most pathogenic gastrointestinal nematodes of small ruminants. The current diagnostic approach for the detection of this species relies on coproscopic methods, which both have low sensitivity and are time consuming. Methods employing detection through DNA amplification, such as droplet digital polymerase chain reaction (ddPCR), offer an advantageous approach to the diagnosis of H. contortus. However, DNA extraction protocols need to be constantly updated for the optimal retrieval of diagnostically usable template. Here, we describe the evaluation of three genomic DNA extraction kits for the detection and quantification of H. contortus ITS2 amplicon DNA from faecal samples, using droplet digital PCR.
resultsDNA samples, extracted from faecal material with the Nucleospin DNA Stool kit, produced the highest amounts of ITS2 amplicon copies and had the lowest coefficient of variation across different dilutions and sample types (fresh or frozen) out of the tested kits (Nucleospin DNA Stool, E.Z.N.A.
conclusionsThe Nucleospin DNA Stool kit is an attractive option for the detection and quantification of H. contortus DNA in faecal samples of small ruminants in a diagnostic setting.
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