ArticleCurrent biology : CB2022
Naegleria's mitotic spindles are built from unique tubulins and highlight core spindle features.
Article in Current biology : CB, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Power-law scaling of mitotic spindles with genome sizes across eukaryotes is driven by chromosome crowding.Nature cell biology · 2026Article
- Role of α-tubulin helix 11' in heterodimer conformation and microtubule dynamics.bioRxiv : the preprint server for biology · 2026Article
- Evolutionary cell biology comes of age.Journal of cell science · 2025Article
- Myosin 2 drives actin contractility in fast-crawling species outside of the amorphean lineage.Current biology : CB · 2025Article
- Tubulin sequence divergence is associated with the use of distinct microtubule regulators.Current biology : CB · 2025Article
- Computational discovery of potential therapeutic agents against brain-eating amoeba (Naegleria fowleri).PloS one · 2025Article
- Torques within and outside the human spindle balance twist at anaphase.The Journal of cell biology · 2024Article
- Article
- An internally controlled system to study microtubule network diversification links tubulin evolution to the use of distinct microtubule regulators.bioRxiv : the preprint server for biology · 2024Article
- Transcriptomic profiling of "brain-eating amoeba"Frontiers in cellular and infection microbiology · 2024Article
- Torques within and outside the human spindle balance twist at anaphase.bioRxiv : the preprint server for biology · 2023Article
- Evaluation and validation of reference genes for RT-qPCR gene expression in Naegleria gruberi.Scientific reports · 2023Article
- A conserved pressure-driven mechanism for regulating cytosolic osmolarity.Current biology : CB · 2023Article
- Mechanisms underlying spindle assembly and robustness.Nature reviews. Molecular cell biology · 2023Review
- Review
- A conserved pressure-driven mechanism for regulating cytosolic osmolarity.bioRxiv : the preprint server for biology · 2023Article
- The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin.Current biology : CB · 2022Article
- The Amazing Evolutionary Complexity of Eukaryotic Tubulins: Lessons fromFrontiers in cell and developmental biology · 2022Article
- Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles.Biophysical journal · 2021Article
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10 authors at 4 institutions in 2 countries.
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Abstract
Naegleria gruberi is a unicellular eukaryote whose evolutionary distance from animals and fungi has made it useful for developing hypotheses about the last common eukaryotic ancestor. Naegleria amoebae lack a cytoplasmic microtubule cytoskeleton and assemble microtubules only during mitosis and thus represent a unique system for studying the evolution and functional specificity of mitotic tubulins and the spindles they assemble. Previous studies show that Naegleria amoebae express a divergent α-tubulin during mitosis, and we now show that Naegleria amoebae express a second mitotic α- and two mitotic β-tubulins. The mitotic tubulins are evolutionarily divergent relative to typical α- and β-tubulins and contain residues that suggest distinct microtubule properties. These distinct residues are conserved in mitotic tubulin homologs of the "brain-eating amoeba" Naegleria fowleri, making them potential drug targets. Using quantitative light microscopy, we find that Naegleria's mitotic spindle is a distinctive barrel-like structure built from a ring of microtubule bundles. Similar to those of other species, Naegleria's spindle is twisted, and its length increases during mitosis, suggesting that these aspects of mitosis are ancestral features. Because bundle numbers change during metaphase, we hypothesize that the initial bundles represent kinetochore fibers and secondary bundles function as bridging fibers.
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