ArticleApplied biochemistry and biotechnology2022
Biochemical Characterization of Thermostable Carboxymethyl Cellulase and β-Glucosidase from Aspergillus fumigatus JCM 10253.
Article in Applied biochemistry and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed, 39 citations in OpenAlex.
- Green biocatalysis: Box-Behnken-optimized cellulase from thermophilic Streptomyces griseorubens NBR14 for waste management and dye removal.Biodegradation · 2026Article
- Comparative Enzymology and Biomass Hydrolysis Reveal Industrial Biorefining Potential ofMicroorganisms · 2026Article
- Diversity and epimedium biotransformation potential of cultivable endophytic fungi associated with Epimedium brevicornum Maxim in the Qinling Mountains, China.Microbial cell factories · 2025Article
- Powerful cell wall biomass degradation enzymatic system from saprotrophicCell surface (Amsterdam, Netherlands) · 2024Review
- Biochemical andHeliyon · 2024Article
- Enhancing the Heterologous Expression of a Thermophilic Endoglucanase and Its Cost-Effective Production inInternational journal of molecular sciences · 2023Article
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3 authors at 2 institutions in 1 country.
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Abstract
Second-generation biofuel production has emerged as a prominent sustainable and alternative energy. The biochemical properties of cellulolytic enzymes are imperative for cellulosic biomass conversion into fermentable sugars. In the present study, thermostable CMCase and β-glucosidase were purified and characterized from Aspergillus fumigatus JCM 10253. The enzymes were purified through 80% ammonium sulfate precipitation, followed by dialysis and DEAE-cellulose ion-exchange chromatography. The molecular masses of the purified CMCase and β-glucosidase were estimated to be 125 kDa and 90 kDa, respectively. The CMCase and β-glucosidase demonstrated optimum activities at pH 6.0 and 5.0, respectively. Their respective maximum temperatures were 50 and 60 °C. The cellulase activities were stimulated by 10 mM concentration of Ca
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