ArticleClinical oral investigations2022
The antimicrobial effect of Rosmarinus officinalis extracts on oral initial adhesion ex vivo.
Article in Clinical oral investigations, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 43 citations in OpenAlex.
- Therapeutic Potential of Latin American Medicinal Plants in Oral Diseases: From Dental Pain to Periodontal Inflammation-A Systematic Review.International journal of molecular sciences · 2025Pooled it
- Microbiological and Clinical Short-Term Evaluation of the Efficacy of an Herbal Tincture as an Adjunctive Treatment in the Management of Stage II, Grade A Periodontitis.Pathogens (Basel, Switzerland) · 2025Trial
- Balancing the Oral Redox State: Endogenous and Exogenous Sources of Reactive Oxygen Species and the Antioxidant Role of Lamiaceae and Asteraceae.Dentistry journal · 2025Review
- Medicinal Plants Against Dental Caries: Research and Application of Their Antibacterial Properties.Plants (Basel, Switzerland) · 2025Review
- Evaluation of early bacterial adhesion on CAD/CAM dental materials: an in situ study.Odontology · 2025Article
- Antibiofilm potential of plant extracts: inhibiting oral microorganisms andFrontiers in dental medicine · 2025Article
- Antibiotic-potentiating efficacy ofFrontiers in pharmacology · 2025Article
- Plant polyphenols, terpenes, and terpenoids in oral health.Open medicine (Warsaw, Poland) · 2025Review
- Exploring the therapeutic potential of medicinal plants and their active principles in dental care: A comprehensive review.Heliyon · 2024Review
- Plant-Derived Compounds: A Promising Tool for Dental Caries Prevention.Current issues in molecular biology · 2024Review
- Optimizing the use of low-frequency ultrasound for bacterial detachment of in vivo biofilms in dental research-a methodological study.Clinical oral investigations · 2023Article
- The oral microbiota is a reservoir for antimicrobial resistance: resistome and phenotypic resistance characteristics of oral biofilm in health, caries, and periodontitis.Annals of clinical microbiology and antimicrobials · 2023Article
- Overview of strategies to improve the antibacterial property of dental implants.Frontiers in bioengineering and biotechnology · 2023Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveIn the last few decades, there has been a growing worldwide interest in the use of plant extracts for the prevention of oral diseases. The main focus of this interest lies in the identification and isolation of substances that limit the formation of microbial biofilm which plays a major role in the development of caries, periodontitis, and peri-implantitis. In this clinical ex vivo study, we investigated the antimicrobial effects of Rosmarinus officinalis extract against oral microorganisms within in situ initial oral biofilms. MATERIALS AND
methodsInitial in situ biofilm samples (2 h) from six healthy volunteers were treated ex vivo with R. officinalis extract at concentrations of 20 mg/ml and 30 mg/ml. The number of viable bacterial cells was determined by counting the colony-forming units. All surviving bacteria were isolated in pure cultures and identified using MALDI-TOF and biochemical testing procedures. Additionally, live/dead staining in combination with epifluorescence microscopy was used for visualizing the antimicrobial effects in the initial biofilms.
resultsThe number of colony-forming units in the R. officinalis-treated biofilms was significantly lower than in the untreated controls (p < 0.001). The reduction range of log10 was 1.64-2.78 and 2.41-3.23 for aerobic and anaerobic bacteria, respectively. Regarding the bacterial composition, large intra- and interindividual variability were observed. Except for Campylobacter spp., the average amount of all bacterial taxa was lower after treatment with R. officinalis than in the untreated biofilms. A total of 49 different species were detected in the untreated biofilms, while only 11 bacterial species were detected in the R. officinalis-treated biofilms. Live/dead staining confirmed that the R. officinalis-treated biofilms had significantly lower numbers of surviving bacteria than the untreated biofilms.
conclusionsThe treatment with R. officinalis extract has a significant potential to eliminate microbial oral initial biofilms. CLINICAL RELEVANCE: The results of this study encourage the use of R. officinalis extracts in biofilm control and thus in the treatment of caries and periodontitis as a herbal adjuvant to synthetic substances.
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