ArticleJournal of clinical laboratory analysis2022
A simple and reliable bile acid assay in human serum by LC-MS/MS.
Article in Journal of clinical laboratory analysis, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- Clinical Relevance of Bile Acid and Bile Acid-Related Therapy in Liver Cirrhosis: A Review.Advances in therapy · 2026Review
- Development and application of a fluorescent immunochromatographic assay for metabolite G-DCA quantification in ICP.Journal of lipid research · 2026Article
- Towards the clinical use of peripheral bile acids: Recommendations to limit their preanalytical and analytical sources of variability.Practical laboratory medicine · 2025Article
- Rapid quantification of murine bile acids using liquid chromatography-tandem mass spectrometry.Analytical and bioanalytical chemistry · 2025Article
- LC-MS/MS identifies elevated imidazole propionate and gut-derived metabolite alterations in peritoneal dialysis patients.Computational and structural biotechnology journal · 2025Article
- Quantitative Determination of a Series of Oxysterols by an Optimized LC-MS/MS Analysis in Different Tissue Types.International journal of molecular sciences · 2024Article
- Alteration of serum bile acids in non-small cell lung cancer identified by a validated LC-MS/MS method.Journal of cancer research and clinical oncology · 2023Article
- Investigation of the potential role of TGR5 in pancreatic cancer by a comprehensive molecular experiments and the liquid chromatography mass spectrometry (LC-MS) based metabolomics.Discover oncology · 2022Article
- A simple and reliable bile acid assay in human serum by LC-MS/MS.Journal of clinical laboratory analysis · 2022Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBile acids, as important signaling molecules and regulatory factors acting on glucose, lipid, and energy metabolism, are always involved in liver, biliary, and intestinal diseases. Development and validation of a simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determination of bile acids is significant for the routine clinical testing.
methodsFifty microlitre of serum was mixed with 10 μl of the internal standard working solution and then 140 μl of methanol for protein precipitation. After centrifuged, the supernatant was directly used for LC-MS/MS analysis.
resultsGood separation of all bile acid species was achieved. The method was validated with consistent linearity for individual bile acids, good recovery, low carryover, satisfactory sample stability, and analytical specificity against hemolysis, lipemia, and bilirubinemia. The intra-day and the inter-day imprecision values were in the range of 1.53%-10.63% and 3.01%-13.98%, respectively. No obvious matrix effect was observed. The reference intervals of bile acids in adults have been established for the clinical testing.
conclusionsThe low sample volume, simple sample preparation, good separation of all species, and satisfying validation results make this LC-MS/MS approach suitable for usage as a high-throughput assay in routine clinical laboratories.
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