ArticleScientific reports2022
Synthesis of two novel bio-based hydrogels using sodium alginate and chitosan and their proficiency in physical immobilization of enzymes.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 48 citations in OpenAlex.
- Spreadable Biosensing Pregel for Analyte Visualization in Peeled Plant Tissues.Analytical chemistry · 2026Article
- Alginate and Chitosan-Based Hydrogels for the Treatment of Radiation Dermatitis.International wound journal · 2026Review
- Enhanced stability and reusability of metagenomic laccase via immobilization on functionalized mesoporous silica for antibiotic contaminant removal.Scientific reports · 2026Article
- Preparation, Characterization, and In Vitro Digestion Behavior of Alginate-Chitosan Microspheres Loaded withFoods (Basel, Switzerland) · 2026Article
- Immobilization of purified pectinase from Aspergillus nidulans on chitosan and alginate beads for biotechnological applications.Microbial cell factories · 2025Article
- Carboxylated nanocellulose from quinoa husk for enhanced protease immobilization and stability of protease in biotechnological applications.Scientific reports · 2025Article
- Microbial Upcycling of Waste PET to Adipic Acid.ACS central science · 2023Article
- Programmable Polyproteams of Tyrosine Ammonia Lyases as Cross-Linked Enzymes for SynthesizingBiomolecules · 2022Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Herein, four novel and bio-based hydrogel samples using sodium alginate (SA) and chitosan (CH) grafted with acrylamide (AAm) and glycidyl methacrylate (GMA) and their reinforced nanocomposites with graphene oxide (GO) were synthesized and coded as SA-g-(AAm-co-GMA), CH-g-(AAm-co-GMA), GO/SA-g-(AAm-co-GMA), and GO/CH-g-(AAm-co-GMA), respectively. The morphology, net charge, and water absorption capacity of samples were entirely changed by switching the biopolymer from SA to CH and adding a nano-filler. The proficiencies of hydrogels were compared in the immobilization of a model metagenomic-derived xylanase (PersiXyn9). The best performance was observed for GO/SA-g-poly(AAm-co-GMA) sample indicating better stabilizing electrostatic attractions between PersiXyn9 and reinforced SA-based hydrogel. Compared to the free enzyme, the immobilized PersiXyn9 on reinforced SA-based hydrogel showed a 110.1% increase in the released reducing sugar and almost double relative activity after 180 min storage. While immobilized enzyme on SA-based hydrogel displayed 58.7% activity after twelve reuse cycles, the enzyme on CH-based carrier just retained 8.5% activity after similar runs.
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