ArticleScientific reports2022
Optimizing intact skull intrinsic signal imaging for subsequent targeted electrophysiology across mouse visual cortex.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Characterizing optogenetically mediated rebound effects in anaesthetized mouse primary visual cortex.The Journal of physiology · 2025Article
- Lateral inhibition in V1 controls neural and perceptual contrast sensitivity.Nature neuroscience · 2025Article
- Highly selective visual receptive fields in mouse frontal cortex.bioRxiv : the preprint server for biology · 2025Article
- Environmental context influences visual processing in thalamus.Current biology : CB · 2025Article
- High-Sensitivity Intrinsic Optical Signal Imaging Through Flexible, Low-Cost Adaptations of an Upright Microscope.eNeuro · 2023Article
- Narrowband gamma oscillations propagate and synchronize throughout the mouse thalamocortical visual system.Neuron · 2023Article
- Intrinsic, widefield optical imaging of hemodynamics in rodent models of Alzheimer's disease and neurological injury.Neurophotonics · 2023Review
- Cranial and Spinal Window Preparation forExperimental neurobiology · 2022Review
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Authors and funding
8 authors.
Funding
Abstract
Understanding brain function requires repeatable measurements of neural activity across multiple scales and multiple brain areas. In mice, large scale cortical neural activity evokes hemodynamic changes readily observable with intrinsic signal imaging (ISI). Pairing ISI with visual stimulation allows identification of primary visual cortex (V1) and higher visual areas (HVAs), typically through cranial windows that thin or remove the skull. These procedures can diminish long-term mechanical and physiological stability required for delicate electrophysiological measurements made weeks to months after imaging (e.g., in subjects undergoing behavioral training). Here, we optimized and directly validated an intact skull ISI system in mice. We first assessed how imaging quality and duration affect reliability of retinotopic maps in V1 and HVAs. We then verified ISI map retinotopy in V1 and HVAs with targeted, multi-site electrophysiology several weeks after imaging. Reliable ISI maps of V1 and multiple HVAs emerged with ~ 60 trials of imaging (65 ± 6 min), and these showed strong correlation to local field potential (LFP) retinotopy in superficial cortical layers (r
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