ArticleScientific reports2022
Irreversible alteration of extracellular vesicle and cell-free messenger RNA profiles in human plasma associated with blood processing and storage.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- MIBlood-EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research.Journal of extracellular vesicles · 2023Pooled it
- A high-throughput, end-to-end pipeline for extracellular miRNA biomarker discovery from human biofluids.Cell reports methods · 2026Article
- The Proteo-Transcriptome of Extracellular Vesicles and Particles Is Largely Preserved After Cryopreservation.Journal of extracellular biology · 2026Article
- Automated Mag-Net Enrichment Unlocks Deep and Cost-Effective LC-MS Plasma Proteomics.Journal of proteome research · 2026Article
- Quantification of EV-associated miRNA in liquid biopsies for biomarker signature development.Extracellular vesicles and circulating nucleic acids · 2026Review
- Cholesterol Deficiency Directs Autophagy-Dependent Secretion of Extracellular Vesicles.Journal of extracellular vesicles · 2026Article
- Extracellular RNAs as Messengers and Early Biomarkers in Neurodegeneration.International journal of molecular sciences · 2025Review
- Cell free RNA detection of pancreatic cancer in pre diagnostic high risk and symptomatic patients.Nature communications · 2025Article
- Cholesterol deficiency directs autophagy-dependent secretion of extracellular vesicles.bioRxiv : the preprint server for biology · 2025Article
- Choice of blood collection methods influences extracellular vesicles counts and miRNA profiling.Journal of extracellular biology · 2024Article
- Storage Stability of Blood Samples for miRNAs in Glycosylated Extracellular Vesicles.Molecules (Basel, Switzerland) · 2023Article
- Circulating extracellular vesicles in healthy and pathological pregnancies: A scoping review of methodology, rigour and results.Journal of extracellular vesicles · 2023Article
- Extracellular vesicles in systemic juvenile idiopathic arthritis.Journal of leukocyte biology · 2023Article
- Selective enrichment of plasma cell-free messenger RNA in cancer-associated extracellular vesicles.Communications biology · 2023Article
- Small extracellular vesicles are released ex vivo from platelets into serum and from residual blood cells into stored plasma.Journal of extracellular biology · 2023Article
- Emerging role of extracellular vesicles in multiple sclerosis: From cellular surrogates to pathogenic mediators and beyond.Journal of neuroimmunology · 2023Review
- Platelet removal from human blood plasma improves detection of extracellular vesicle-associated miRNA.Journal of extracellular vesicles · 2023Article
- Integrated analysis of circulating cell free nucleic acids for cancer genotyping and immune phenotyping of tumor microenvironment.Frontiers in genetics · 2023Review
- Current challenges and best practices for cell-free long RNA biomarker discovery.Biomarker research · 2022Review
- Effects of ex vivo blood anticoagulation and preanalytical processing time on the proteome content of platelets.Journal of thrombosis and haemostasis : JTH · 2022Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
The discovery and utility of clinically relevant circulating biomarkers depend on standardized methods that minimize preanalytical errors. Despite growing interest in studying extracellular vesicles (EVs) and cell-free messenger RNA (cf-mRNA) as potential biomarkers, how blood processing and freeze/thaw impacts the profiles of these analytes in plasma was not thoroughly understood. We utilized flow cytometric analysis to examine the effect of differential centrifugation and a freeze/thaw cycle on EV profiles. Utilizing flow cytometry postacquisition analysis software (FCMpass) to calibrate light scattering and fluorescence, we revealed how differential centrifugation and post-freeze/thaw processing removes and retains EV subpopulations. Additionally, cf-mRNA levels measured by RT-qPCR profiles from a panel of housekeeping, platelet, and tissue-specific genes were preferentially affected by differential centrifugation and post-freeze/thaw processing. Critically, freezing plasma containing residual platelets yielded irreversible ex vivo generation of EV subpopulations and cf-mRNA transcripts, which were not removable by additional processing after freeze/thaw. Our findings suggest the importance of minimizing confounding variation attributed to plasma processing and platelet contamination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.