Evidence map›Paper›PMID 35135890›Full record

ArticleCold Spring Harbor protocols2022

Oral RNAi for Gene Silencing in Mosquitoes: From the Bench to the Field.

Keshava Mysore, Limb K Hapairai, Jacob S Realey, Longhua Sun, Joseph B Roethele, Molly Duman-Scheel

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cold Spring Harbor protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 7 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Keshava Mysore *Department of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, Indiana 46617, USA.
Limb K Hapairai *Department of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, Indiana 46617, USA.
Jacob S RealeyDepartment of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, Indiana 46617, USA.
Longhua SunDepartment of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, Indiana 46617, USA.
Joseph B RoetheleDepartment of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, Indiana 46617, USA.
Molly Duman-ScheelDepartment of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, Indiana 46617, USA; mscheel@nd.edu.
Indiana University · USIndiana University School of MedicineUniversity of Notre Dame · US

Funding

Development of Yeast Interfering RNA Larvicides Targeting Aedes aegyptiR21AI128116 · NIAID · INDIANA UNIVERSITY INDIANAPOLIS · PI SCHEEL, MOLLY DUMAN, SEVERSON, DAVID W · 2017 to 2018
$444k
NIAID NIH HHS R21 AI128116
6 · The paper itself

Abstract

RNA interference (RNAi) has played a key role in the field of insect functional genomics, a discipline that has enhanced the study of developmental, evolutionary, physiological, and molecular biological phenomena in a wide variety of insects, including disease vector mosquitoes. Here we introduce a recently optimized RNAi procedure in which adult mosquitoes are fed with a colored sugar bait containing small interfering RNA (siRNA). This procedure effectively and economically leads to gene silencing, is technically straightforward, and has been successfully used to characterize a number of genes in adult mosquitoes. We also discuss how, in addition to laboratory applications, this oral RNAi procedure might one day be used in the field for controlling insect pests.

Indexed as

CulicidaeAnimalsGene SilencingInsectaMosquito VectorsRNA, Double-StrandedRNA InterferenceRNA, Small InterferingRNA, Double-StrandedRNA, Small Interfering

Identifiers

PMID35135890
PMCPMC11041366
OpenAlexW4211108828

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.