Evidence map›Paper›PMID 35134701›Full record

ArticleBiomaterials2022

Point-of-care treatment of geometrically complex midfacial critical-sized bone defects with 3D-Printed scaffolds and autologous stromal vascular fraction.

Srujan Singh, Ethan L Nyberg, Aine N O'Sullivan, Ashley Farris, Alexandra N Rindone, Nicholas Zhang, Emma C Whitehead, Yuxiao Zhou, Eszter Mihaly, Chukwuebuka C Achebe and 10 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
5.6field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 26 citations in OpenAlex.

  1. Article
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  5. Review
  6. Article
  7. 3D printed OActa biomaterialia · 2024
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 4 institutions in 1 country.

Srujan SinghDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA; Translational Tissue Engineering Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Ethan L NybergTranslational Tissue Engineering Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Aine N O'SullivanTranslational Tissue Engineering Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Ashley FarrisTranslational Tissue Engineering Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Alexandra N RindoneTranslational Tissue Engineering Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Nicholas ZhangTranslational Tissue Engineering Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Emma C WhiteheadTranslational Tissue Engineering Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Yuxiao ZhouTranslational Tissue Engineering Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Eszter MihalyTranslational Tissue Engineering Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Chukwuebuka C AchebeTranslational Tissue Engineering Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Wojciech ZbijewskiTranslational Tissue Engineering Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Will GrundyAlizée Pathology, LLC, A StageBio Company, Thurmont, MD, USA.
David GarlickAlizée Pathology, LLC, A StageBio Company, Thurmont, MD, USA.
Nicolette D JacksonAlizée Pathology, LLC, A StageBio Company, Thurmont, MD, USA.
Takashi TaguchiDepartment of Veterinary Clinical Sciences, Louisiana State University, Baton Rouge, LA, USA.
Catherine TakawiraDepartment of Veterinary Clinical Sciences, Louisiana State University, Baton Rouge, LA, USA.
Joseph LopezDivision of Plastic Surgery, Yale-New Haven Hospital, New Haven, CT, USA.
Mandi J LopezDepartment of Veterinary Clinical Sciences, Louisiana State University, Baton Rouge, LA, USA.
Michael P GrantDepartment of Plastic & Reconstructive Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Plastic & Reconstructive Surgery, R Adams Cowley Shock Trauma Center, University of Maryland School of Medicine, Baltimore, MD, USA.
Warren L GraysonDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA; Translational Tissue Engineering Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD, USA; Institute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, USA. Electronic address: wgrayson@jhmi.edu.
Johns Hopkins University · USLouisiana State University · USUniversity of Maryland, Baltimore · USYale New Haven Hospital · US

Funding

Oxygen-eluting scaffolds for cranial bone regenerationR01DE027957 · NIDCR · JOHNS HOPKINS UNIVERSITY · PI GRAYSON, WARREN L, PATHAK, ARVIND P · 2019 to 2023
$2.4M
Evaluating blood vessel phenotype in tissue-engineered craniofacial bone grafts using quantitative 3D light-sheet microscopyF31DE029109 · NIDCR · JOHNS HOPKINS UNIVERSITY · PI RINDONE, ALEXANDRA · 2019 to 2020
$92k
Investigating the Role of Hypoxic Signaling on Adipose-Derived Stem Cell OsteogenesisF31AR075368 · NIAMS · JOHNS HOPKINS UNIVERSITY · PI FARRIS, ASHLEY · 2020 to 2021
$74k
NIAMS NIH HHS F31 AR075368NIDCR NIH HHS F31 DE029109NIDCR NIH HHS R01 DE027957
6 · The paper itself

Abstract

Critical-sized midfacial bone defects present a unique clinical challenge due to their complex three-dimensional shapes and intimate associations with sensory organs. To address this challenge, a point-of-care treatment strategy for functional, long-term regeneration of 2 cm full-thickness segmental defects in the zygomatic arches of Yucatan minipigs is evaluated. A digital workflow is used to 3D-print anatomically precise, porous, biodegradable scaffolds from clinical-grade poly-ε-caprolactone and decellularized bone composites. The autologous stromal vascular fraction of cells (SVF) is isolated from adipose tissue extracts and infused into the scaffolds that are implanted into the zygomatic ostectomies. Bone regeneration is assessed up to 52 weeks post-operatively in acellular (AC) and SVF groups (BV/DV = 0.64 ± 0.10 and 0.65 ± 0.10 respectively). In both treated groups, bone grows from the adjacent tissues and restores the native anatomy. Significantly higher torque is required to fracture the bone-scaffold interface in the SVF (7.11 ± 2.31 N m) compared to AC groups (2.83 ± 0.23 N m). Three-dimensional microcomputed tomography analysis reveals two distinct regenerative patterns: osteoconduction along the periphery of scaffolds to form dense lamellar bone and small islands of woven bone deposits growing along the struts in the scaffold interior. Overall, this study validates the efficacy of using 3D-printed bioactive scaffolds with autologous SVF to restore geometrically complex midfacial bone defects of clinically relevant sizes while also highlighting remaining challenges to be addressed prior to clinical translation.

Indexed as

Stromal Vascular FractionTissue ScaffoldsAnimalsBone RegenerationOsteogenesisPoint-of-Care SystemsPrinting, Three-DimensionalSwineSwine, MiniatureX-Ray Microtomography3D printingCritical-sized bone defectsDecellularized boneStromal vascular fractionYucatan pigs

Identifiers

PMID35134701
PMCPMC12619679
OpenAlexW4210412979

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.