ArticleThe Science of the total environment2022
Comparison of virus concentration methods and RNA extraction methods for SARS-CoV-2 wastewater surveillance.
Article in The Science of the total environment, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
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Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 112 citations in OpenAlex.
- Recent progress on wastewater-based epidemiology for COVID-19 surveillance: A systematic review of analytical procedures and epidemiological modeling.The Science of the total environment · 2023Pooled it
- A Wastewater-based Modeling Framework for Inferring Norovirus Transmission Dynamics.Environmental science & technology · 2026Article
- An integrated framework for early detection and transmissibility assessment of emerging variants in wastewater.International journal of hygiene and environmental health · 2026Article
- Environmental and microbial factors shaping SARS-CoV-2 RNA decay in wastewater: insights from batch tests and a lab-scale sewer pipeline simulator.Scientific reports · 2026Article
- Effect of nucleic acid concentration method on Severe Acute Respiratory Syndrome Coronavirus 2 detection sensitivity in aircraft wastewater.Journal of medical microbiology · 2026Article
- Utilizing wastewater surveillance to model behavioural responses and prevent healthcare overload during "Disease X" outbreaks.Emerging microbes & infections · 2025Article
- Coupling wastewater-based epidemiology with data-driven machine learning for managing public health risks.Risk analysis : an official publication of the Society for Risk Analysis · 2025Article
- Assess the Variability and Robustness of an Aluminum-Based Adsorption-Precipitation Method for Virus Detection in Wastewater Samples.Microorganisms · 2025Article
- Trends and Challenges in the Detection and Environmental Surveillance of the Hepatitis E Virus.Microorganisms · 2025Review
- The Impact of Viral Concentration Method on Quantification and Long Amplicon Nanopore Sequencing of SARS-CoV-2 and Noroviruses in Wastewater.Microorganisms · 2025Article
- Review
- Consistent trends from different methods for monitoring SARS-CoV-2 in urban wastewater during a 29-month longitudinal study.Frontiers in microbiology · 2025Article
- Performance of protein N linear epitopes in serodiagnosis of COVID-19 infection.BioImpacts : BI · 2025Article
- A Narrative Review of High Throughput Wastewater Sample Processing for Infectious Disease Surveillance: Challenges, Progress, and Future Opportunities.International journal of environmental research and public health · 2024Review
- The effects of RT-qPCR standards on reproducibility and comparability in monitoring SARS-CoV-2 levels in wastewater.Scientific reports · 2024Article
- Article
- Evaluation of Commercial RNA Extraction Protocols for Avian Influenza Virus Using Nanopore Metagenomic Sequencing.Viruses · 2024Article
- Comparison of Different Reverse Transcriptase-Polymerase Chain Reaction-Based Methods for Wastewater Surveillance of SARS-CoV-2: Exploratory Study.JMIR public health and surveillance · 2024Article
- Article
- Surveillance of influenza A and B viruses from community and hospital wastewater treatment plants.Environmental microbiology reports · 2024Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wastewater surveillance is a promising tool for population-level monitoring of the spread of infectious diseases, such as the coronavirus disease 2019 (COVID-19). Different from clinical specimens, viruses in community-scale wastewater samples need to be concentrated before detection because viral RNA is highly diluted. The present study evaluated eleven different virus concentration methods for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in wastewater. First, eight concentration methods of different principles were compared using spiked wastewater at a starting volume of 30 mL. Ultracentrifugation was the most effective method with a viral recovery efficiency of 25 ± 6%. The second-best option, AlCl
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.