ArticleBMC genomics2022
The potential use of mitochondrial ribosomal genes (12S and 16S) in DNA barcoding and phylogenetic analysis of trematodes.
Article in BMC genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 37 citations in OpenAlex.
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- Morphological and molecular identification of the cat fleaJournal of advanced veterinary and animal research · 2025Article
- Unveiling Tick Diversity in Cattle in Cameroon: Emergence ofVeterinary sciences · 2025Article
- Helminths infection of Schizothorax niger in Kashmir, India: morphological and molecular characterization.Molecular biology reports · 2025Article
- Internal transcribed spacers enable species-level Metataxonomic analysis of ciliated protozoa.ISME communications · 2025Article
- Genetic differentiation of Oncomelania hupensis robertsoni in hilly regions of China: Using the complete mitochondrial genome.PLoS neglected tropical diseases · 2024Article
- Evaluation of unmodified human cell-derived extracellular vesicle mitochondrial deoxyribonucleic acid-based biodistribution in rodents.Journal of extracellular vesicles · 2024Article
- DNA barcoding for the identification and authentication of medicinal deer (Cervus sp.) products in China.PloS one · 2024Article
- Discovery ofParasitology · 2024Article
- High-throughput DNA metabarcoding for determining the gut microbiome of captive critically endangered Malayan tiger (Biodiversity data journal · 2023Article
- Sensitive and accurate DNA metabarcoding of parasitic helminth mock communities using the mitochondrial rRNA genes.Scientific reports · 2022Article
- What lies behind the curtain: Cryptic diversity in helminth parasites of human and veterinary importance.Current research in parasitology & vector-borne diseases · 2022Review
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5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGenetic markers like the nuclear ribosomal RNA (rRNA) genes, internal transcribed spacer regions, mitochondrial protein-coding genes, and genomes have been utilized for molecular identification of parasitic trematodes. However, challenges such as the design of broadly applicable primers for the vast number of species within Digenea and the genetic markers' ability to provide sufficient species-level resolution limited their utility. This study presented novel and broadly applicable primers using the mitochondrial 12S and 16S rRNA genes for Digenea and aimed to show their suitability as alternative genetic markers for molecular identification of orders Plagiorchiida, Echinostomida, and Strigeida.
resultsOur results revealed that the mitochondrial 12S and 16S rRNA genes are suitable for trematode molecular identification, with sufficient resolution to discriminate closely related species and achieve accurate species identification through phylogenetic placements. Moreover, the robustness of our newly designed primers to amplify medically important parasitic trematodes encompassing three orders was demonstrated through successful amplification. The convenience and applicability of the newly designed primers and adequate genetic variation of the mitochondrial rRNA genes can be useful as complementary markers for trematode molecular-based studies.
conclusionsWe demonstrated that the mitochondrial rRNA genes could be alternative genetic markers robust for trematode molecular identification and potentially helpful for DNA barcoding where our primers can be widely applied across the major Digenea orders. Furthermore, the potential of the mitochondrial rRNA genes for molecular systematics can be explored, enhancing their appeal for trematode molecular-based studies. The novelty of utilizing the mitochondrial rRNA genes and the designed primers in this study can potentially open avenues for species identification, discovery, and systematics in the future.
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