ArticleVeterinary pathology2022
Perfusion with 10% neutral-buffered formalin is equivalent to 4% paraformaldehyde for histopathology and immunohistochemistry in a mouse model of experimental autoimmune encephalomyelitis.
Article in Veterinary pathology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 16 citations in OpenAlex.
- Fenofibrate Mitigates Acute Lung Injury in a Rat Model of Feces-Induced Peritonitis.International journal of molecular sciences · 2026Article
- Brain extraction for fixed tissue banking: a technical report.Free neuropathology · 2026Article
- Unveiling the Toxic Mechanism of Arsenic on the Spleen of Cyprinus carpio and the Antagonistic Role of Zinc via the P38 MAPK/Nrf2/HO- 1 Pathway.Biological trace element research · 2025Article
- Article
- Immunological findings of West Caucasian bat virus in an accidental host.Journal of virology · 2025Article
- Evaluating ultrastructural preservation quality in banked brain tissue.Free neuropathology · 2025Article
- Whole-Mount Immunohistochemical and Immunofluorescence Assays in Zebrafish Embryos.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Spontaneous early-onset neurodegeneration in the brainstem and spinal cord of NSG, NOG, and NXG mice.Veterinary pathology · 2023Article
- Effect of induced diabetes on morphometric indexes of the cerebellar cortex and gene expression in C57BL mice.Iranian journal of basic medical sciences · 2023Article
- [Importance of intracardiac perfusion fixation technique forRevista cientifica odontologica (Universidad Cientifica del Sur)Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
Intravascular (IV) perfusion of tissue fixative is commonly used in the field of neuroscience as the central nervous system tissues are exquisitely sensitive to handling and fixation artifacts which can affect downstream microscopic analysis. Both 10% neutral-buffered formalin (NBF) and 4% paraformaldehyde (PFA) are used, although IV perfusion with PFA is most commonly referenced. The study objective was to compare the severity of handling and fixation artifacts, semiquantitative scores of inflammatory and neurodegenerative changes, and quantitative immunohistochemistry following terminal IV perfusion of mice with either 10% NBF or 4% PFA in a model of experimental autoimmune encephalitis (EAE). The study included 24 mice; 12 were control animals not immunized and an additional 12 were immunized with PLP
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.