ArticlePLoS neglected tropical diseases2022
Evaluation of a simple, rapid and field-adapted diagnostic assay for enterotoxigenic E. coli and Shigella.
Article in PLoS neglected tropical diseases, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT07433426 (The Impact of Shigellosis and Recommended Treatment in Children), which is not on this map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
The Impact of Shigellosis and Recommended Treatment in Children
Who cites it
12 citing papers in PubMed, 16 citations in OpenAlex.
- Antimicrobial resistance and toxin profiles of enterotoxigenic Escherichia coli in children under five years old with diarrhoea in Ouagadougou, Burkina Faso: a sentinel surveillance study.BMC infectious diseases · 2026Article
- Evaluation of the performance and feasibility of RLDT in detectingmedRxiv : the preprint server for health sciences · 2025Article
- Can Wastewater Surveillance Enhance Genomic Tracking of Climate-Driven Pathogens?Microorganisms · 2025Review
- Occurrence of enterotoxigenicFrontiers in environmental science · 2025Article
- Field evaluation of a simple and rapid diagnostic test, RLDT to detect Shigella and enterotoxigenic E. coli in Indian children.Scientific reports · 2024Article
- Epidemiology of EnterotoxigenicMicroorganisms · 2024Article
- Epidemiology of enterotoxigenicFrontiers in public health · 2024Article
- Contribution of the Rapid LAMP-Based Diagnostic Test (RLDT) to the Evaluation of EnterotoxigenicMicroorganisms · 2023Article
- Field evaluation of a simple and rapid diagnostic test, RLDT to detect Shigella and enterotoxigenic E. coli in Indian children.Research square · 2023Article
- Clinical Prediction Rule to Guide Diagnostic Testing forOpen forum infectious diseases · 2023Article
- Development of a simple, rapid, and sensitive molecular diagnostic assay for cholera.PLoS neglected tropical diseases · 2023Article
- Field evaluation of a novel, rapid diagnostic assay, and molecular epidemiology of enterotoxigenic E. coli among Zambian children presenting with diarrhea.PLoS neglected tropical diseases · 2022Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
Abstract
Understanding the global burden of enterotoxigenic E. coli (ETEC) and Shigella diarrhea as well as estimating the cost effectiveness of vaccines to control these two significant pathogens have been hindered by the lack of a diagnostic test that is rapid, simple, sensitive, and can be applied to the endemic countries. We previously developed a simple and rapid assay, Rapid Loop mediated isothermal amplification based Diagnostic Test (RLDT) for the detection of ETEC and Shigella spp. (Shigella). In this study, the RLDT assay was evaluated in comparison with quantitative PCR (qPCR), culture and conventional PCR for the detection of ETEC and Shigella. This validation was performed using previously collected stool samples from endemic countries, from the travelers to the endemic countries, as well as samples from a controlled human infection model study of ETEC. The performance of RLDT from dried stool spots was also validated. RLDT resulted in excellent sensitivity and specificity compared to qPCR (99% and 99.2% respectively) ranging from 92.3 to 100% for the individual toxin genes of ETEC and 100% for Shigella. Culture was less sensitive compared to RLDT. No significant differences were noted in the performance of RLDT using samples from various sources or stool samples from moderate to severe diarrhea or asymptomatic infections. RLDT performed equally well in detection of ETEC and Shigella from the dried stool samples on filter papers. This study established that RLDT is sufficiently sensitive and specific to be used as a simple and rapid diagnostic assay to detect ETEC and Shigella in endemic countries to determine disease burden of these pathogens in the national and subnational levels. This information will be important to guide public health and policy makers to prioritize resources for accelerating the development and introduction of effective preventative and/or treatment interventions against these enteric infections.
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Registered trials
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